Fern gametophytes in culture—a simple system for studying plant development and reproductionDyer, A. F.
doi: 10.1080/00219266.1983.9654495pmid: N/A
Gametophytes are easily cultured, even with limited facilities. They can he used to illustrate not only the alternation of generations in plant life cycles but also aspects of reproduction and development not easily studied with other plant material. The approach can be adjusted to the needs of biology and botany classes over a wide range of experience from secondary school to university. Information is presented on: the choice of species; the collection, storage and sowing of spores; the composition of the medium; light and temperature requirements; sampling and staining techniques; and methods for determining quantitative parameters of cell and gametophyte development. The basic fern life cycle, and modifications such as apogamy, are outlined. Attention is drawn to the main features of the reproductive biology of ferns and appropriate investigations are suggested. The advantages of gametophytes as experimental material for studying cellular aspects of development in intact green plants are explained. They include striking but consistent responses to a range of cultural conditions and environmental treatments. Suggestions are given for investigations to stimulate further discussion of a wide range of developmental events.