Journal of Clinical Microbiology

Hebert, G A; Tzianabos, T; Gamble, W C; Chappell, W A

doi: N/Apmid: 6769955

Rabbits were inoculated with purified antigen preparations of Coxiella burnetii and representative species of the spotted fever and typhus groups of rickettsiae. Their antibody responses were monitored by complement fixation tests; high-titered antisera were fractionated with ammonium sulfate and then labeled with fluorescein isothiocyanate by the dialysis method. The conjugates had homologous 3+ staining titers of 1:256 to 1:2,048 and did not exhibit nonspecific staining. The Rickettsia rickettsii, R. conorii, and R. akari conjugates reacted only with rickettsiae of the spotted fever group; the R. canada, R. prowazekii, and R. typhi conjugates were specific for the typhus group rickettsiae; and the C. burnetii conjugate stained only homologous organisms. One of these conjugates (R. rickettsii) is currently being used to identify rickettsiae in clinical specimens and has already proven its value as a diagnostic tool. J Clin Microbiol. 1980 May; 11(5): 503-507

Blanchette, E A; Rubin, S J

doi: N/Apmid: 7381012

The distribution of capsular serotypes of 200 clinical isolates of Klebsiella pneumoniae and Klebsiella oxytoca from four Connecticut hospitals was determined. Serotyping was done by an indirect fluorescent-antibody technique. Hospitals included three community hospitals from the Hartford area and one university hospital in New Haven. During the test period, epidemiological surveillance did not detect any nosocomial epidemic involving Klebsiella species. Ninety-two percent of the isolates were typable. Of the 72 possible serotypes, 62 were represented among these strains. Forty-two percent of the typable strains were distributed among 10 serotypes. The predominant serotypes were types 31, 22, and 18 representing 19% of the typable strains (8, 6, and 5%, respectively). No one particular serotype was associated exclusively with a specific site of infection. J Clin Microbiol. 1980 May; 11(5): 474-478

Parkinson, A J; Muchmore, H G; Scott, L V; Miles, J A

doi: N/Apmid: 6247369

Using a portable minaturized cell culture system, enhanced recoveries of parainfluenza virus types 1 and 3 were made in the field from symptomatic human adult subjects working at remote Antarctic stations. J Clin Microbiol. 1980 May; 11(5): 535-536

Kurien, S; Lorian, V

doi: N/Apmid: 7381021

Discrepancies between results obtained by agar and broth techniques in testing of drug combinations. S Kurien and V Lorian ABSTRACT One hundred strains of Staphylococcus epidermidis were tested by the double-disk agar diffusion method for their susceptibility to cephalothin, to sulfamethoxazole-trimethoprim, and to combinations of these drugs. Forty-five strains showed a bridging inhibition zone between the two disks, suggesting a synergistic effect of the combination of cephalothin plus sulfamethoxozole-trimethoprim against these strains. Ten of these strains were retested against the individual drugs and their combination by the twofold broth dilution method. No strain showed higher susceptibility to any combination as compared with cephalothin alone. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. May 1980 vol. 11 no. 5 527-529 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Kurien, S. Articles by Lorian, V. Search for related content PubMed PubMed citation Articles by Kurien, S. Articles by Lorian, V. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • [email protected] Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info»

Hébert, G A; Tzianabos, T; Gamble, W C; Chappell, W A

doi: N/Apmid: 6769955

Development and characterization of high-titered, group-specific fluorescent-antibody reagents for direct identification of rickettsiae in clinical specimens. G A Hébert , T Tzianabos , W C Gamble and W A Chappell ABSTRACT Rabbits were inoculated with purified antigen preparations of Coxiella burnetii and representative species of the spotted fever and typhus groups of rickettsiae. Their antibody responses were monitored by complement fixation tests; high-titered antisera were fractionated with ammonium sulfate and then labeled with fluorescein isothiocyanate by the dialysis method. The conjugates had homologous 3+ staining titers of 1:256 to 1:2,048 and did not exhibit nonspecific staining. The Rickettsia rickettsii, R. conorii, and R. akari conjugates reacted only with rickettsiae of the spotted fever group; the R. canada, R. prowazekii, and R. typhi conjugates were specific for the typhus group rickettsiae; and the C. burnetii conjugate stained only homologous organisms. One of these conjugates (R. rickettsii) is currently being used to identify rickettsiae in clinical specimens and has already proven its value as a diagnostic tool. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. May 1980 vol. 11 no. 5 503-507 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Hébert, G. A. Articles by Chappell, W. A. Search for related content PubMed PubMed citation Articles by Hébert, G. A. Articles by Chappell, W. A. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • [email protected] Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info»

Chester, B; Poulos, E G

doi: N/Apmid: 6991527

Each of 28 Vibrio strains and 3 Campylobacter strains was immobilized in distilled water (pH 6.9), but retained full motility in Trypticase soy broth. This phenomenon was not seen with any of 243 non-Vibrio, non-Campylobacter isolates representing 44 taxa of gram-negative bacteria. J Clin Microbiol. 1980 May; 11(5): 537-539

Syed, S A

doi: N/Apmid: 7381020

Gram-negative, non-saccharolytic, brown- or black-pigment-forming, nonmotile anaerboic coccobacilli, capable of decomposing hydrogen peroxide and identified as Bacteroides asaccharolyticus (B. melaninogenicus subsp. asaccharolyticus), were isolated from the supra- and subgingival plaques of beagle dogs with gingivitis or periodontitis. The organisms remained viable for many hours in an aerobic atmosphere as evidenced by their ability to grow subsequently in an anaerobic environment. They also grew well on agar media that were not reduced before use. Although blood was required for pigmentation of colonies, organisms grew on media that lacked hemin, menadione, blood, or reducing compounds. Increased oxygen tolerance, catalase activity, and different nutritional requirements differentiate these organisms from strains of B. asaccharolyticus isolated from humans. J Clin Microbiol. 1980 May; 11(5): 522-526

Wong, M; Catena, A; Hadley, W K

doi: N/Apmid: 6769956

Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-reactions in heterologous antigen-antibody combinations were eliminated, leaving only species-specific precipitin arcs. These absorbed antisera, specific for each Peptostreptococcus species by Ouchterlony tests, were used for rapid identification studies. Staphylococcus aureus-bearing protein A was sensitized with each absorbed antiserum. These reagents produced specific coagglutination reactions with suspensions of each Peptostreptococcus reference strain and with 16 clinical isolates. No cross-reactions occurred with the Streptococcus intermedius, Peptococcus magnus, or Peptococcus asaccharolyticus strains tested. J Clin Microbiol. 1980 May; 11(5): 515-521

Boughton, W H; Atkin, J F

doi: N/Apmid: 7381022

Ventricular peritoneal shunt infection caused by a member of the rhodochrous complex. W H Boughton and J F Atkin ABSTRACT Microorganisms of the rhodochrous complex were isolated in pure culture from a ventricular peritoneal shunt in a 5.5-month-old child. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. May 1980 vol. 11 no. 5 533-534 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Boughton, W. H. Articles by Atkin, J. F. Search for related content PubMed PubMed citation Articles by Boughton, W. H. Articles by Atkin, J. F. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • [email protected] Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info»

Wong, M; Catena, A; Hadley, W K

doi: N/Apmid: 6769956

Antigenic relationships and rapid identification of Peptostreptococcus species. M Wong , A Catena and W K Hadley ABSTRACT Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-reactions in heterologous antigen-antibody combinations were eliminated, leaving only species-specific precipitin arcs. These absorbed antisera, specific for each Peptostreptococcus species by Ouchterlony tests, were used for rapid identification studies. Staphylococcus aureus-bearing protein A was sensitized with each absorbed antiserum. These reagents produced specific coagglutination reactions with suspensions of each Peptostreptococcus reference strain and with 16 clinical isolates. No cross-reactions occurred with the Streptococcus intermedius, Peptococcus magnus, or Peptococcus asaccharolyticus strains tested. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. May 1980 vol. 11 no. 5 515-521 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Wong, M. Articles by Hadley, W. K. Search for related content PubMed PubMed citation Articles by Wong, M. Articles by Hadley, W. K. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • [email protected] Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info»