Stem Cells

doi: 10.1002/stem.5530110901pmid: 7905317

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Frassoni, Francesco; Carella, Angelo M.

doi: 10.1002/stem.5530110902pmid: 8298470

Autografting, Chronic myeloid leukemia References 1 Goldman JM , Catowsky , D, Hows J, Spiers ASD, Galton DAG. Cryopreserved peripheral blood cells functioning as autografts in patients with chronic granulocytic leukaemia in transformation . Br Med J 1979 ; 1 : 1310 – 1313 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Corin NC , Aegerter P, Auvert B, Meloni G, Goldstone AH, Burnett A, Carella A, Korbling M, Herve P, Maraninchi D, Löwenberg R, Verdonck LF, de Planque M, Hermans J, Helbig W, Porcellini A, Rizzoli V, Alesandrino EP, Franklin IM, Reiffers J, Colleselli P, Goldman JM. Autologous bone marrow transplantation for acute myelocytic leukemia in first remission: a European survey of the role of marrow purging . Blood 1990 ; 75 : 1606 – 1614 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 3 Gorin NC , Labopin M, Meloni G, et al. Autologous bone marrow transplantation for acute myeloblastic leukemia in Europe: further evidence of the role of marrow purging by mafosfamide . Leukemia 1992 ; 6 : 367 – 374 Google Scholar OpenURL Placeholder Text WorldCat 4 Carella AM , Frassoni F, Van Lint MT, et al. Autologous and allogeneic BMT in acute myeloid leukemia in first remission: an update of the Genoa experience with 1569 patients . Ann Hematol 1992 ; 64 : 128 – 133 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Horowitz MM , Gale RP, Somdel PM, Goldman JM, Kersey J, Kolb H-J, Rimm AA, Ringdén O, Rozman C, Speck B, Truitt RL, Zwaan FE, Bortin MM. Graft-versus-leukemia reactions after marrow transplantation . Blood 1990 ; 75 : 555 – 562 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Goldman JM , Gale RP, Horowitz MM, et al. Bone marrow transplantation for chronic myelogenous leukemia in chronic phase: increased risk of relapse associated with T-cell depletion . Ann Intern Med 1988 ; 108 : 806 – 814 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Gale RP , Butturini A. How do marrow transplants cure leukemia? in RE Champlin and RP Gale eds. New Strategies in Bone Marrow Transplantation . Wiley-Liss, Inc. 1991 ; 109 – 118 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 8 Gale RP , Butturini A. How do transplants cure chronic myelogenic leukaemia? . Bone Marrow Transplant 1992 ; 9 : 83 – 85 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 9 Jones RJ , Volgesang GB, Hess AD. Induction of graft-versus-host disease after autologous bone marrow transplantation . Lancet 1989 ; 1 : 754 – 756 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Coulombel L , Kaulosek DK, Eaves CJ, et al. Long-term marrow culture reveals chromosomally normal hemopoietic progenitor cells in patients with Philadelphia chromosome positive chronic myelogenous leukemia . N Engl J Med 1983 ; 308 : 1493 – 1498 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Brito-Babapulle F , Bowcock SJ, Marcus RE, et al. Autografting for patients with chronic myeloid leukaemia in chronic phase: peripheral blood stem cells may have a finite capacity for maintaining hemopoiesis . Br J Haematol 1989 ; 73 : 76 – 81 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Frassoni F , Sessarego M, Bacigalupo A, et al. Competition between recipient and donor cells after marrow transplantation for chronic myeloid leukaemia . Br J Haemotol 1985 ; 69 , 471 – 475 . Google Scholar Crossref Search ADS WorldCat 13 Eaves CJ , Eaves AC. Cell culture studies in CML . Bailliere's Clinical Haematology 1987 ; 1 : 931 . Google Scholar Crossref Search ADS WorldCat 14 Frassoni F , Repetto M, Podesta M, et al. Competitive survival/proliferation of normal and Ph-positive hematopietic cells . Br J Haematol 1986 ; 63 : 135 – 141 . Google Scholar Crossref Search ADS PubMed WorldCat 15 Barnett MJ , Eaves CJ, Philips GL, et al. Successful autografting in chronic myeloid leukemia after maintenance of marrow culture . Bone Marrow Transplant 1989 ; 4 : 345 – 351 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Article PDF first page preview Close This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Goldman, John M.; O'Brien, Stephen G.

doi: 10.1002/stem.5530110903pmid: 8298477

Abstract Some patients with Philadelphia (Ph) hromosome-positive chronic myeloid leukemia CML) may achieve partially or completely Ph-negtive hemopoiesis after treatment with high doses of ytotoxic drugs or interferon-α at standard dosage. This observation leads to three important questions: 1) are Ph-negative myeloid cells in such patients strictly normal? 2) can such Ph-negative ells be identified in all newly diagnosed patients or only in a minority? and 3) what is the basis for he proliferative advantage manifested by CML ells and why might it be temporarily lost as a result of treatment? The mechanisms that might prevent he proliferation of normal cells in an environment of Ph-positive cells and the relevance of these questions to the design of a strategy aimed at obtaining complete remission in the majority of patients are considered. Such a strategy might incorporate auto-grafting with Ph-negative stem cells harvested during the recovery phase of high dose chemotherapy. Chronic myeloid leukemia, Normal stem cells, Normal progenitors, Autografting References 1 Martiat P , Michaux JL Rodhain J. Philadelphia-negative chronic myeloid leukemia: comparison with Ph+ CML and chronic myelomonocytic leukemia . Blood 1991 ; 78 : 205 – 211 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Cogswell PC , Morgan R, Dunn M, Neubauer A, Nelson P, Poland-Johnson NK, Sandberg AA, Liu E. Mutations of the ras protooncogenes in chronic myelogenous leukemia: a high frequency of ras mutations in bcr/abl rearrangement-negative chronic myelogenous leukemia . Blood 1989 ; 74 : 2629 – 2633 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Fialkow PJ , Martin PJ, Najfeld V, Penfold GK, Jacobson RJ, Hansen JA. Evidence for a multistep pathogenesis of chronic myelogenous leukemia . Blood 1981 ; 58 : 158 – 163 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Ferraris AM , Canepa L, Melani C, Miglino M, Broccia G, Gaetani GF. Clonal B lymphocytes lack bcr rearrangement in Ph-positive chronic myelogenous leukaemia . Br J Haematol 1989 ; 73 : 48 – 50 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Lisker R , Casas L, Mutchinick O, Perez-Chavez F, Labardini J. Late-appearing Philadelphia chromosome in two patients with chronic myelogenous leukemia . Blood 1980 ; 56 : 812 – 814 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Finney R , McDonald GA, Baikie AG. Chronic granulocytic leukaemia with Ph-negative cells in bone marrow and a ten year remission after busulfan hypoplasia . Br I Haematol 1972 ; 23 : 283 – 288 . Google Scholar Crossref Search ADS WorldCat 7 Weinberger A , Benjamin D, Douer D. A 13 year remission in chronic myelocytic leukaemia after a single course of busulfan . Acta Haematol 1978 ; 59 : 354 – 359 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Smalley RV , Vogel J, Huguley CM, Miller D. Chronic granulocytic leukemia: cytogenetic conversion of the bone marrow with cycle-specific chemotherapy . Blood 1977 ; 50 : 107 – 113 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Sharp JC , Joyner MV, Wayne AW, Kemp J, Crofts M, Birch AD, McArthur G, Lai S, Sterndale H, Williams Y. Karyotypic conversion in Ph1-positive chronic myeloid leukaemia with combination chemotherapy . Lancet 1979 ; 1 : 1370 – 1372 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Cunningham I , Gee T, Dowling M. Results of treatment of Ph1 positive chronic myeloid leukemia with an intensive treatment regimen (L-5 protocol) . Blood 1979 ; 53 : 375 – 395 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Goto T , Nishikori M, Arlin Z, Gee T, Kempin S, Burchenal J, Strife A, Wisniewski D, Lambek C, Little C, Jhanwar S, Chaganti R, Clarkson B. Growth characteristics of leukemic and normal hemopoietic cells in Ph+ chronic myelogenous leukemia and effects of intensive treatment . Blood 1982 ; 59 : 793 – 808 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Talpaz M , Kantarjian H, Kurzrock R. Interferon-alpha produces sustained cytogenetic responses in chronic myelogenous leukemia . Ann Intern Med 1991 ; 114 : 532 – 538 . Google Scholar Crossref Search ADS PubMed WorldCat 13 Chervenick PA , Ellis LD, Pan SF, Lawson AL. Human leukemic cells: in vitro growth of colonies containing the Philadelphia (Ph1) chromosome . Science 1971 ; 174 : 1134 – 1136 . Google Scholar Crossref Search ADS PubMed WorldCat 14 Coulombel L , Kalousek DK, Eaves CH, Gupta CM, Eaves AC. Long term marrow culture reveals chromosomally normal hematopoietic progenitor cells in patients with Philadelphia chromosome-positive chronic myelogenous leukemia . New Engl J Med 1983 ; 306 : 1493 – 1498 . Google Scholar OpenURL Placeholder Text WorldCat 15 Hogge DE , Coulombel L, Kalousek DK, Eaves CJ, Eaves AC. Nonclonal hemopoietic progenitors in a G6PD heterozygote with chronic myelogenous leukemia revealed after long-term marrow culture . Am J Hematol 1987 ; 24 : 389 – 394 . Google Scholar Crossref Search ADS PubMed WorldCat 16 Butturini A , Keating A, Goldman JM, Gale RP. Autotransplants in chronic myelogenous leukaemia: strategies and results . Lancet 1990 ; 335 : 1255 – 1258 . Google Scholar Crossref Search ADS PubMed WorldCat 17 Haines ME , Goldman JM, Worsley AM, McCarthy DM, Wyatt SE, Dowding C, Kearney L, Th'ng KH, Wareham NJ, Pollock A. Chemotherapy and autografting for chronic granulocytic leukaemia in transformation: probable prolongation of survival in some patients . Br J Haematol 1984 ; 58 : 711 – 721 . Google Scholar Crossref Search ADS PubMed WorldCat 18 Reiffers J , Trouette R, Marit G. Autologous blood stem cell transplantation for chronic granulocytic leukaemia in transformation: a report of 47 cases . Br J Haematol 1991 ; 77 : 339 – 345 . Google Scholar Crossref Search ADS PubMed WorldCat 19 Brito-Babapulle F , Bowcock SJ, Marcus RE, Apperley J, Th'ng KH, Dowding C, Rasool F, Guo Ai-Pu, Catovsky D, Galton D, McCarthy D, Goldman JM. Autografting for patients with chronic myeloid leukaemia in chronic phase: peripheral blood stem cells may have a finite capacity for maintaining haemopoiesis . Br J Haematol 1989 ; 73 : 76 – 81 . Google Scholar Crossref Search ADS PubMed WorldCat 20 Korbling M , Burke P, Braine H, Elfenbein G, Santos G, Kaizer H. Successful engraftment of blood derived stem cells in chronic myelogenous leukemia . Exp Hematol 1981 ; 9 : 684 – 690 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 21 Carella AM , Gaozza E, Raffo MR, Carlier P, Frassoni F, Valbonesi M, Lercari G, Sessarego M, Deffarrari R, Guerrasio A, Saglio G, Canepa L, Gaetani GF, Occhini D. Therapy of acute phase chronic myelogenous leukaemia with intensive chemotherapy, blood cell autotransplant and cyclosporine A . Leukemia 1991 ; 5 : 517 – 521 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 22 Daley GQ , Van Etten RA, Baltimore D. Blast crisis in a murine model of chronic myelogenous leukemia . Proc Nat Acad Sci USA 1991 ; 88 : 11335 – 11338 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Gishizky ML , Johnson-White J, Witte ON. Efficient transplantation of BCR-ABL induced chronic myelogenous leukemia-like syndrome in mice . Proc Nat Acad Sci USA 1993 ; 90 : 3755 – 3759 . Google Scholar Crossref Search ADS PubMed WorldCat 24 Otsuka T , Eaves CJ, Humphries RK, Hogge DE, Eaves AC. Lack of evidence for abnormal autocrine or paracrine mechanisms underlying the uncontrolled proliferation of primitive chronic myeloid leukaemia progenitor cells . Leukemia 1991 ; 5 : 861 – 868 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 25 Dunlop DJ , Wright EG, Lorimore S, Graham GJ, Holyoake T, Kerr DJ, Wolpe SD, Pragnell IB. Demonstration of stem cell inhibition and myelo-protective effect of SCI/rhMIP 1α in vivo . Blood 1992 ; 79 : 2221 – 2225 . Google Scholar Crossref Search ADS PubMed WorldCat 26 Daley GQ , Goldman JM. Autologous transplant for CML revisited . Exp Hematol 1993 ; 21 : 734 – 737 . Google Scholar PubMed OpenURL Placeholder Text WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Kantarjian, Hagop M.; Talpaz, Moshe

doi: 10.1002/stem.5530110904pmid: 8298480

Chronic myelogenous leukemia, Therapy, Alpha interferon, Bone marrow transplantation References 1 Daley GQ , Van Etten RA, Baltimore D. Induction of chronic myelogenous leukemia by the P210 bcr/abl gene of the Philadelphia chromosome . Science 1990 ; 247 : 824 – 829 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Goldman JM , Grosveld G, Baltimore D, Gale RP. Chronic myelogenous leukemia—the unfolding saga . Leukemia 1990 ; 4 : 163 – 167 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 3 McGlave PB . Therapy of chronic myelogenous leukemia with related or unrelated donor bone marrow transplantation . Leukemia 1992 ; 6 : 115 – 117 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 4 Goldman JM , McGlave P, Szydlo P, Gale RP, Horowitz MM. Impact of disease duration and prior treatment on outcome of bone marrow transplants for chronic myelogenous leukemia . Blood 1992 ; 80 ( suppl 1 ): 170a . Google Scholar OpenURL Placeholder Text WorldCat 5 Zuffa E , for the Italian Cooperative Study Group on Chronic Myeloid Leukemia . The Italian prospective study of interferon-alpha (Roferon) vs. chemotherapy—karyotypic response and survival at 4 years. In: Chronic Myeloid Leukemia, 2nd International Conference, Bologna, Italy, 1992 ; 163 . 6 Kantarjian HM , Keating MJ, Estey EH, O'Brien S, Pierce S, Beran M, Roller C, Feldman E. Talpaz M. Treatment of advanced stages of Philadelphia chromosome-positive chronic myelogenous leukemia with interferon-α and low-dose cytarabine . J Clin Oncol 1992 ; 10 : 772 – 778 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Guilhot F , Tanzer J, Bautrs F, et al. A multicenter randomized study of alpha 2b interferon and hydroxyurea with or without cytosine-arabinoside in previously untreated patients with Ph+ CML . Haematologica 1991 ; 76 : 64 . Google Scholar OpenURL Placeholder Text WorldCat 8 O'Brien S , Kantarjian H, Beran M, et al. Homoharringtonine produces high response rates in Philadelphia chromosome positive chronic myelogenous leukemia . Blood 1991 ; 78 ( suppl 1 ): 170a . Google Scholar OpenURL Placeholder Text WorldCat 9 O'Brien S , Kantarjian H, Feldman E, et al. Homoharringtonine produces high hematologic and cytogenetic response rates in Philadelphia chromosome positive chronic myelogenous leukemia . Leukemia 1992 ; 6 : 118 – 119 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Barnett MJ , Eaves CJ, Phillips GL, et al. Autografting in chronic myeloid leukemia with cultured marrow . Leukemia 1992 ; 6 : 118 – 119 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 11 Deisseroth A , Herst V, Wedrychowski A, et al. Future directions in molecular and genetic therapy for leukemias and solid tumors . Can Bull 1990 ; 43 : 173 – 180 . Google Scholar OpenURL Placeholder Text WorldCat 12 Carella AM , Gaozza E, Raffo MR, et al. Therapy of acute phase chronic myelogenous leukemia with intensive chemotherapy, blood cell auto-transplant and cyclosporine A . Leukemia 1991 ; 5 : 517 – 521 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Article PDF first page preview Close This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Mcglave, Philip; Verfaillie, Catherine; Miller, Jeffrey

doi: 10.1002/stem.5530110905pmid: 7905318

Abstract Donor marrow transplantation can cure chronic myelogenous leukemia (CML). Unfortunately, the procedure is associated with severe complications and is limited to the minority of potential recipients with suitably matched donors. Autologous marrow transplantation using negative selection approaches such as incubation with gamma interferon (IFN-γ) can produce cytogenetic and clinical remissions, but they are often associated with recurrent evidence of leukemia. A primitive progenitor population can be separated from normal human marrow on the basis of morphologic characteristics and cell surface antigen expression. Cell populations with similar morphologic and phenotypic characteristics obtained by positive selection from the marrow of patients with CML appear to be benign. Benign primitive and committed progenitors selected in this fashion can be expanded ex vivo when cultured in a “Transwell” system which physically separates hematopoietic cells from stromal feeder layers. Positive selection and ex vivo cultivation of benign progenitors from CML marrow may provide a source of hematopoietic stem cells suitable for autologous marrow transplantation. Autologous natural killer (NK) cells obtained from the peripheral blood of patients with CML are of benign origin and have antileukemia activity. Interleukin 2 (IL-2) activated autologous NK cells may be used in post-transplant cellular therapy to prevent recurrence of CML. Chronic myelogenous leukemia, Stem cells, Autologous transplant, Primitive progenitors, Positive selection, CD34 References 1 Thomas ED , Clift RA, Fefer A, Appelbaum FR, Beatty P, Bensinger WI, Buckner CD, Cheever MA, Deeg HJ, Doney K, Flournoy N, Greenberg P, Hansen JA, Martin P, McGuffin R, Ramberg R, Sanders JE, Singer J, Stewart P, Storb R, Sullivan K, Weiden PL, Witherspoon RW. Marrow transplantation for the treatment of chronic myelogenous leukemia . Ann Intern Med 1986 ; 104 : 155 – 163 . Google Scholar Crossref Search ADS PubMed WorldCat 2 McGlave P , Bartsch G, Anasetti C, Ash R, Beatty P, Gajewski J, Kernan N. Unrelated donor bone marrow transplantation therapy for chronic myelogenous leukemia: initial experience of the National Marrow Donor Program . Blood , 1993 ; 81 [ 2 ]: 543 – 550 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Dubé ID , Gupta CM, Kalousek DK, Eaves CJ, Eaves AC. Cytogenetic studies of early myeloid progenitor compartments in Ph1-positive chronic myeloid leukaemia-persistence of Ph1-negative committed progenitors that are suppressed from differentiating in vivo . Br J Haematol 1984 ; 56 : 633 – 644 . Google Scholar Crossref Search ADS PubMed WorldCat 4 McGlave PB , Mamus S, Vilen B, Dewald G. Effect of recombinant gamma interferon on chronic myelogenous leukemia bone marrow progenitors . Exp Hematol 1987 ; 15 : 331 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 McGlave P , Arthur D, Miller W, Lasky L, Kersey J. Autologous transplantation for CML using marrow treated ex vivo with recombinant human interferon gamma . Bone Marrow Transplantation 1990 ; 6 : 115 – 120 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 6 Verfaillie C , Blakolmer K, McGlave P. Purified primitive hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiate bone marrow stroma . J Exp Med 1990 ; 172 : 509 – 520 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Verfaillie CM , Miller WJ, Boyland K, McGlave PB. Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression . Blood 1992 ; 79 : 1003 – 1011 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Verfaillie CM . Direct contact between progenitors and stroma is not required for human in vitro hematopoiesis . Blood 1992 ; 92 : 2821 – 2826 . Google Scholar OpenURL Placeholder Text WorldCat 9 Verfaillie CM , Myhre KL, Miller JS, McGlave PB. Selection and in vitro expansion of benign, primitive progenitors in early chronic phase CML suitable for transplantation . Blood 1993 ; 80 : 358a . Google Scholar OpenURL Placeholder Text WorldCat 10 Verfaillie C , Kay N, Miller W, McGlave P. Adherent lymphokine activated killer (ALAK) cells in chronic myelogenous leukemia: a benign cell population with potent cytotoxic activity . Blood 1989 ; 74 : 793 – 797 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Verfaillie C , Kay N, Miller W, McGlave P. Diminished A-LAK cytotoxicity and proliferation accompanies disease progression in chronic myelogenous leukemia . Blood 1990 ; 76 : 401 – 408 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Miller JS , Oelkers S, Verfaillie C, McGlave P. The role of monocytes in the expansion of human activated natural killer cells . Blood , 1992 ; 80 : 2221 – 2229 . Google Scholar Crossref Search ADS PubMed WorldCat 13 McGlave PB , Arthur D, Haake R, Hurd D, Miller W, Vercellotti G, Weisdorf D, Kim T, Ramsay NKC, Kersey J. Therapy of chronic myelogenous leukemia with allogeneic bone marrow transplantation . J Clin Oncol 1987 ; 5 : 1033 – 1040 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Gulati, S.; Lemoli, R.; Fraig, M.

doi: 10.1002/stem.5530110906pmid: 7905321

Abstract Allogeneic bone marrow transplantation (BMT) is a useful approach for treating patients with chronic myelogenous leukemia (CML). In patients who lack a suitable donor, various approaches to utilize the patient's own hematopoietic stem cells obtained from bone marrow and/or peripheral blood have been developed. Preferential recovery of normal human hematopoietic progenitors over CML clone in long-term bone marrow culture (LTBMC) has been observed for a long time. LTBMC initiated in tissue culture (TC) flasks to that in “Lifecell” bags, which are gas-permeable plastic bags in which feeder-layer cells cannot adhere, has been investigated for several years. In our laboratory, cells were incubated in the presence of various growth factors to develop improved methods for stem cell expansion. Sustained hematopoietic stem cell growth in the absence of a feeder layer in plastic gas-permeable bags has been observed. Growth of marrow from patients with CML is being investigated extensively. Combining effective drugs to decrease the Philadelphia (Ph) clone prior to bone marrow harvest and the use of cultured bone marrow may provide a useful method for treating patients with CML. Results of various international studies are also discussed below. CML, LTBMC, BM culture References 1 Tura S , Russo D, Zaffa E, Fiacchini M, Baccarani M. A prospective comparison of α-IFN and conventional chemotherapy in Ph+ chronic myeloid leukemia. Clinical and cytogenetic results at 2 years in 322 patients . Haematol 1992 ; 77 : 204 – 214 . Google Scholar OpenURL Placeholder Text WorldCat 2 Hehlmann R , Heimpel H, Heinze B, Kolb H-J, Wussow PV, Thiele J, Georgii A, Hochhaus A, Ansari H, Hasford J. Chronic myelogenous leukemia: recent developments in prognostic evaluation and chemotherapy . Leukemia 1992 ; 6 :(Suppl 3)110S-114S. Google Scholar OpenURL Placeholder Text WorldCat 3 Marks DI , Hughes TP, Szydlo R, Kelly S, Cullis JO, Schwarer AP, Mackinnon S, Apperley J, Barrett AJ, Hows JM, Goldman JM. HLA-identical sibling donor bone marrow transplantation for chronic myeloid leukaemia in first chronic phase: influence of GVHD prophylaxis on outcome . Br J Haematol 1992 ; 81 : 383 – 390 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Wagner JE , Zahurak M, Piantadosi S, Geller RB, Vogelsang GB, Wingard JR, Saral R, Griffin C, Shah N, Zehnbauer BA, Ambinder R, Burns W, Jones R, May WS, Rowley S, Yeager A, Santos GW. Bone marrow transplantation of chronic myelogenous leukemia in chronic phase: evaluation of risks and benefits . J Clin Oncol 1992 ; 10 : 779 – 789 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Cunningham I , Castro-Malaspina H, Flomengberg N, Gulati SC, Shank B, Collins N, Keever C, O'Reilly RJ. Improved results of bone marrow transplantation for chronic myelogenous leukemia using marrow depleted of T-cells by soybean lectin agglutination and E-rosette depletion. Progress in Bone Marrow Transplantation , Alan R. Liss, Inc. 1987 ; 359 – 363 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 6 Mackinnon S , Hows JM, Goldman JM. Induction of in vitro graft-versus-leukemia activity following bone marrow transplantation for chronic myeloid leukemia . Blood 1990 ; 76 : 2037 – 2045 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Roux E , Abdi K, Speiser D, Helg C, Chapius B, Jeannet M, Rossnek E. Characterization of mixed chimerism in patients with chronic myeloid leukemia transplanted with T-cell-depleted bone marrow: Involvement of different hematologic lineages before and after relapse . Blood 1993 ; 81 : 243 – 248 . Google Scholar Crossref Search ADS PubMed WorldCat 8 McGlave P , Bartsch G, Anasetti C, Ash R, Beatty P, Gajewski J, Kernan NA. Unrelated donor marrow transplantation therapy for chronic myelogenous leukemia: initial experience of the National Marrow Donor Program . Blood 1993 ; 81 : 543 – 550 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Bar BMAM , Schattenberg A, Mensink EJBM, Guerts Van Kessel A, Smetsers TFCM, Knops GHJN, Linders EHP, De Witte T. Donor leukocyte infusions for chronic myeloid leukemia relapsed after allogeneic bone marrow transplantations . J Clin Oncol 1993 ; 11 : 513 – 519 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Higano CS , Raskind WH, Singer JW. Use of a interferon for the treatment of relapse of chronic myelogenous leukemia in chronic phase after allogeneic bone marrow transplantation . Blood 1992 ; 80 : 1437 – 1442 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Coco F , Mandelli F, Diverio D. Therapy-induced Ph1 suppression in chronic myeloid leukemia: molecular and cytogenetic studies in patients treated with alpha-2b IFN, high-dose chemotherapy and autologous stem cell infusion . Bone Marrow Transplantation 1990 ; 62 : 253 – 258 . Google Scholar OpenURL Placeholder Text WorldCat 12 Gartner S , Kaplan HS. Long-term culture of human bone marrow cells . Proc Natl Acad Sci USA 1980 ; 77 : 4756 – 4759 . Google Scholar Crossref Search ADS PubMed WorldCat 13 Coulombel L , Kalousek DK, Eaves CJ, Gupta CM, Eaves AC. Long-term marrow culture reveals chromosomally normal hematopoietic cells in patients with Philadelphia chromosome-positive chronic myelogenous leukemia . New Engl J Med. 1983 ; 308 : 1493 – 1498 . Google Scholar Crossref Search ADS WorldCat 14 Dexter TM , Allen TD, Lajtha LG. Conditions controlling the proliferation of hematopoietic stem cells in vitro . J Cell Physiol 1977 ; 91 : 335 – 344 . Google Scholar Crossref Search ADS PubMed WorldCat 15 Barnett MJ , Eaves CJ, Kalousek DK, Klingermann HG, Lansdorp PM, Reece DE, Shepperd SD, Shaw GH, Eaves AC. Successful autografting in chronic myeloid leukemia after maintenance of marrow in culture . Bone Marrow Transplantation 1989 ; 4 : 345 – 351 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 16 Carella AM , Policardo N, Raffo MR, Podesta M, Carlier P, Valbonesi M, Lercari G, Vitale V, Gallamini A. Intensive conventional chemotherapy can lead to a precocious overshoot of cytogenetically normal blood stem cells (BSC) in chronic myeloid leukemia and acute lymphoblastic leukemia . Leukemia 1992 ; 6 ( Suppl 4 ): 120 – 123 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 17 Stoppa AM , Maraninchi D, Michel G, Carcassonne Y. Liquid culture and cryopreservation of marrow cells of leukaemic patients prior to autologous bone marrow transplantation . Br J Haematol 1989 ; 72 : 519 . Google Scholar Crossref Search ADS PubMed WorldCat 18 Brandt J , Srour EF, van Besien K, Briddel RA, Hoffman R. Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow . J Clin Invest 1990 ; 86 : 932 . Google Scholar Crossref Search ADS PubMed WorldCat 19 Coutinho LH , Will A, Radford J, Schiro' R, Testa NG, Dexter TM. Effects of recombinant human granulocyte colony-stimulating factor (CSF), human granulocyte-macrophage-CSF and gibbon Interleukin-3 on hemopoiesis in human long-term bone marrow culture . Blood 1990 ; 75 : 2118 – 2129 . Google Scholar Crossref Search ADS PubMed WorldCat 20 Lemoli RM , Tafuri A, Strife A, Andreeff M, Clarkson BD, Gulati SC. Proliferation of human hematopoietic progenitors in long-term bone marrow cultures in gas-permeable plastic bags is enhanced by colony-stimulating factors . Exp Hematol 1992 ; 20 : 569 – 575 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 21 Verfaillie CM , McCarthy JB, McGlave PB. Mechanisms underlying abnormal trafficking of malignant progenitors in chronic myelogenous leukemia . J Clin Invest 1992 ; 90 : 1232 – 1241 . Google Scholar Crossref Search ADS PubMed WorldCat 22 Roberts R , Gallagher J, Spooncer E, Allen TD, Bloomfield F, Dexter TM. Heparan sulfate-bound growth factors: A mechanism for stromal cell-mediated hemopoiesis . Nature , 1988 ; 332 : 376 – 378 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Gordon MY . Annotation: Extracellular matrix of the marrow microenvironment . Br J Haematol , 1988 ; 70 : 1 – 4 . Google Scholar Crossref Search ADS PubMed WorldCat 24 Gordon MY , Dowding CR, Riley GP, Goldman JM, Greaves ME. Altered adhesive interactions with marrow stroma of hematopoietic progenitor cells in chronic myeloid leukemia . Nature , 1987 ; 328 : 342 – 344 . Google Scholar Crossref Search ADS PubMed WorldCat 25 Verfaille G , McGlave P. Purification of benign and malignant stem cells in CML . Blood , 1990 ; 76 : 131a . Google Scholar OpenURL Placeholder Text WorldCat 26 Copelan E , Grever M, Kapoor N. Marrow transplantation following busulfan and cyclophosphamide for chronic myelogenous leukaemia in accelerated or blastic phase . Br J Haematol , 1989 ; 71 : 487 – 491 . Google Scholar Crossref Search ADS PubMed WorldCat 27 Szczylik C , Skorski T, Nicolaides NC, Manzella L, Malaguarnera L, Donatella V, Gerwitz AM, Calbretta B. Selective inhibition of leukemia cell proliferation by BCR-ABL antisense oligodeoxynucleotides . Science 1991 ; 253 : 562 – 565 . Google Scholar Crossref Search ADS PubMed WorldCat 28 Leemhuis T , Leibowitz D, Cox G, Silver R, Srour EF, Tricot G, Hoffman R. Identification of bcr/abl-negative primitive hematopoietic progenitor cells within chronic myeloid leukemia marrow . Blood 1993 ; 81 : 801 – 807 . Google Scholar Crossref Search ADS PubMed WorldCat 29 Henon PR , Liang H, Beck-Wirth G, Eisenmann JC, Lepers M, Wunder E, Kandel G. Comparison of hematopoietic and immune recovery after autologous bone marrow or blood stem cell transplants . Bone Marrow Transplantation 1992 ; 9 : 285 – 291 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 30 Melo JV , Gordon DE, Cross NCP, Goldman JM. The abl/bcr fusion gene is expressed in chronic myeloid leukemia . Blood 1993 ; 81 : 158 – 165 . Google Scholar Crossref Search ADS PubMed WorldCat 31 Gulati SC . Purging in bone marrow transplantation , 1993 . Published by R. G. Landes. Distributed by CRC Press , Phone (407) 994–0555, Boca Raton, FL 33431. Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Mughal, Tariq; Hoyle, Christine; Goldman, John M.

doi: 10.1002/stem.5530110907pmid: 8298473

Abstract The optimal approach for curing patients with chronic myeloid leukemia (CML) in chronic phase (CP) is an allogeneic bone marrow transplant BMT). However, this approach is not available to he majority of patients. Autografting, using either burged or unpurged bone marrow or peripheral blood stem cells (PBSC), could be beneficial for patients who are not eligible for BMT. Between 1984 and 1992, 21 patients with CML in CP were auto-grafted using unmanipulated PBSC harvested either at diagnosis or thereafter. Ten of 21 patients survived at a median of 84 months following auto-grafting (range: 12–108 months). Eleven patients lied, seven of blast transformation and four from other causes. The timing of the autograft or the harvest does not appear to have a bearing on survival. Autografts, Chronic myeloid leukemia, Chronic phase References 1 Goldman JM . Bone marrow transplantation for chronic myeloid leukaemia . Bone Marrow Transplant 1991 ; 8 ( suppl 2 ): 62 – 63 . Google Scholar OpenURL Placeholder Text WorldCat 2 Champlin RE , Goldman JM, Gale RP. Bone marrow transplantation in chronic myelogenous leukemia . Semin Hematol 1988 ; 25 : 74 – 80 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 3 McGlave PB , Beatty P, Ash R, Hows JM. Therapy for chronic myelogenous leukemia with unrelated donor bone marrow transplantation: Results in 102 cases . Blood 1990 ; 75 : 1728 – 1732 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Howard MR , Hows JM, Gore SM, Downie T, Bradley BA. Unrelated donor marrow transplantation: An interim report from the IMUST study . Blood 1992 ; 80 ( Suppl 1 ):1324. Google Scholar OpenURL Placeholder Text WorldCat 5 Haines ME , Goldman JM, Worsley AM, McCarthy DM, Wyatt SE, Dowding C, Kearney L, Th'ng KH, Wareham NJ, Pollock A, Galvin MC, Samson D, Geary CG, Catovsky D, Galton DAG. Chemotherapy and autografting for chronic granulocytic leukaemia in transformation: probable prolongation of survival for some patients . Br J Haematol 1984 ; 58 : 711 – 721 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Brito-Babapulle F , Bowcock GJ, Marcus R, Apperley J, Th'ng KH, Dowding C, Rassool F, Ai-Pu G, Catovsky D, Galton D, Goldman J. Results of autografting for patients with chronic myeloid leukaemia in chronic phase . Br J Haematol 1989 ; 73 : 76 – 81 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Goldman JM , Th'ng KH, Park DS, Spiers ASD, Lowenthal RM, Ruutu T. Collection, cryo-preservation and subsequent viability of haemopoietic stem cells intended for treatment of chronic granulocytic leukaemia in transformation . Br J Haematol 1978 ; 40 : 185 – 195 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Brito-Babapulle F , Apperley J, Rassool F, Guo A-P, Dowding C, Goldman JM. Complete remission after autografting for chronic myeloid leukemia . Leuk Res 1987 ; 11 : 1115 – 1117 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Carella AM , Pollicardo N, Raffo NR, Podesta M, Carlier P, Valboniesi M, Lercari G, Vitale V, Gallamini A. Intensive conventional chemotherapy can lead to a precocious overshoot of cytogenetically normal blood stem cells (BSC) in chronic myeloid leukemia and acute lymphoblastic leukemia . Leukemia 1992 ; 6 (suppl 4): 120 – 123 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Simonsson B , Öberg G, Björeman M, Björkholm M, Gahrton G, Hast R, Killander A, Turesson I, Udén AM, Vikrot O, Vilén L, Wahlin A, Carneskog J, Westlin J. Intensive treatment in order to minimize the Ph-positive clone in chronic myelogenous leukemia . Leukemia and Lymphoma 1992 ; 7 : 55 – 57 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Hoyle CM , Gray R, Goldman JM on behalf of the Hammersmith BMT Team . Autografting for patients with CML in chronic phase—an update . Br J Haematol 1993 (In press). 12 Baum CM , Weissman IL, Tsukamoto AS, Buckle AM, Poault B. Isolation of a candidate human hematopoietic stem cell . Proc Natl Acad Sci USA 1992 ; 89 : 2804 – 2808 . Google Scholar Crossref Search ADS PubMed WorldCat 13 De Fabritiis P , Sandrelli A, Meloni G, Alimena G, Montefusco E, Lo Coco F, De Felice I, Mandelli F. Prolonged suppression of myeloid progenitor cell numbers after stopping interferon treatment for CML may necessitate delay in harvesting marrow cells for autografting . Bone Marrow Transplant 1990 ; 6 : 247 – 251 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 14 Deisseroth AB , Korbling M, Reading C, Hester J, Heimfeld S, Talpaz M, Kantarjian H, O'Brien S, Feldman E, Claxton D, Etkin M, Ellerson D, Durett A, Thomas M, Champlin R, Berenson R. Selection and transplantation of CD34+ progenitor cells following retroviral marking in autologous bone marrow transplantation in chronic myeloid leukemia (CML) . Proc ASCO 1993 ; 12 : 987 . Google Scholar OpenURL Placeholder Text WorldCat 15 Kantarjian H , Talpaz M, Reading C, Hester J, Berenson R, Hemfeld S, Korbling M, Liang J, Calvert L, Tibbits P, Thomas M, Durett A, Champlin R, Deisseroth AB. Selective repopulation of peripheral blood with Philadelphia chromosome negative (Ph−) cells after chemotherapy allows collection of engrafting populations of CD34+ Ph-cells in chronic myelogenous leukemia (CML) patients . Proc ASCO 1993 ; 12 : 1018 . Google Scholar OpenURL Placeholder Text WorldCat 16 Calabretta B . Inhibition of protooncogene expression by antisense oligodeoxynucleotides: biological and therapeutic implications . Cancer Res 1991 ; 51 : 4505 – 4510 . Google Scholar PubMed OpenURL Placeholder Text WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Reiffers, J.; Cahn, J. Y.; Montastruc, M.; Caillot, D.; Souillet, G.; Leblond, V.; Troncy, J.; Faberes, C.

doi: 10.1002/stem.5530110908pmid: 7905322

CML, PBSC transplantation, Alpha interferon References 1 Talpaz M , Kantarjian H, Kurzrock R, et al. Interferon-alpha produces sustained cytogenetic responses in chronic myelogenous leukemia . Ann Int Med 1991 ; 114 : 532 – 538 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Talpaz M , Kantarjian HM, McCredie KB, Keating MJ, Trujillo J, Gutterman J. Clinical investigation of human alpha interferon in chronic myelogenous leukemia . Blood 1987 ; 69 : 1280 – 1288 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Sokal JE , Cox EB, Baccarini M, Tura S, Gomez GA, Robertson JE, Tso CY, Braun TJ, Clarkson BD, Cervantes F, Rozman C. Prognostic discrimination in “good risk” chronic granulocytic leukemia . Blood 1984 ; 63 : 789 – 799 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Reiffers J , Meloni G, Goldman J, Gratwohl A on behalf of the EBMT working party on chronic leukemias . Autologous stem cell transplantation for chronic myeloid leukemia: a European Survey. XVIIIth Annual Meeting of the European Group for Bone Marrow Transplantation, Stockholm, May 31-June 3, 1992 . 5 Butturini A , Keating A, Goldman J, et al. Autotransplants in chronic myelogenous leukaemia: strategies and results . Lancet 1990 ; 335 : 1255 – 1258 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Barnett MJ , Eaves CJ, Phillips G, et al. Successful autografting in chronic myeloid leukaemia after maintenance of marrow in culture . Bone Marrow Transplantation 1989 ; 4 : 345 – 351 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Article PDF first page preview Close This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Rizzoli, Vittorio; Mangoni, Lina; Almici, Camillo; Caramatti, Cecilia; Dotti, Gian Pietro; Carlo-Stella, Carmelo

doi: 10.1002/stem.5530110909pmid: 8298474

Abstract Ten adult patients with Ph-positive chronic myelogenous leukemia (CML) received autologous bone marrow transplantation (ABMT) using marrow treated ex vivo with mafosfamide. At the time of ABMT, six patients were in chronic phase and four in accelerated phase. Seven of ten patients reported herein were selected on the basis of a previous laboratory assessment of the numbers of normal and leukemic stroma-adherent progenitor cells within mafosfamide-treated marrow. Only patients showing ≥50% Ph-negative stroma-adherent progenitor cells within mafosfamide-treated marrow were considered eligible for autografting. In nine out of ten evaluable patients, the median time to achieve 500 neutrophils/μl was 32 days (range: 25–72). A platelet count of 2 × 104/μl was achieved at a median of 40 days (range: 27–97). Six out of nine analyzable patients engrafted Ph-negative. The median duration of the Ph-negative hematopoiesis, confirmed also by Southern blot analysis, was 6.5 months (range: 4–30). A good correlation was evident between the results of the in vitro preharvest screening test and the in vivo occurrence of normal hematopoiesis post-transplant. Two patients who showed 75% and 89% Ph-negative stroma-adherent progenitors engrafted Ph-positive, whereas four out of five evaluable patients who had 100% Ph-negative stroma-adherent progenitors engrafted Ph-negative. After a median follow-up of 16 months (range: 3–31), five patients evolved into blast crisis, three are alive in hematologic and cytogenetic relapse, and one died without evolving into blast crisis. In conclusion, our results demonstrate that: 1) engraftment can occur from Ph-negative stem cells selected by mafosfamide purging; 2) in selected CML patients, mafosfamide is effective in reducing the size of the malignant clone and inducing a transient period of Ph-negative hematopoiesis; and 3) modifications of the purging procedure as well as post-transplant manipulation of the immune-hematopoietic system are required to prolong cytogenetic remission or cure CML patients ineligible for allogeneic or unrelated bone marrow transplantation (BMT). CML, ABMT, Marrow purging, Mafosfamide References 1 Fialkow PJ , Gartler SM, Yoshida A. Clonal origin of chronic myelocytic leukemia in man . Proc Natl Acad Sci USA 1987 ; 58 : 1468 – 1471 . Google Scholar Crossref Search ADS WorldCat 2 Koeffler HP , Golde DW. Chronic myelogenous leukemia. New concepts . N Engl J Med 1981 ; 304 : 1269 – 1274 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Kurzrock R , Gutterman JU, Talpaz M. The molecular genetics of Philadelphia chromosome-positive leukemias . N Engl J Med 1988 ; 319 : 990 – 998 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 4 Thomas DE , Clift RA. Indications for marrow transplantation in chronic myelogenous leukemia . Blood 1989 ; 73 : 861 – 864 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Goldman JM . Molecular biology and treatment of chronic myelogenous leukemia . Curr Opin Oncol 1990 ; 2 : 49 – 54 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Goto T , Nishikori M, Arlin Z, Gee T, Kempin S, Burchenal J, Strife A, Wisniewski D, Lambek C, Little C, Jhanwar S, Chaganti R, Clarkson B. Growth characteristics of leukemic and normal hematopoietic cells in Ph+ chronic myelogenous leukemia and effects of intensive treatment . Blood 1982 ; 59 : 793 – 808 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Coulombel L , Kalousek DK, Eaves CJ, Gupta CM, Eaves AC. Long-term marrow culture reveals chromosomally normal hematopoietic progenitor cells in patients with Philadelphia chromosome-positive chronic myelogenous leukemia . N Engl J Med 1983 ; 308 : 1493 – 1498 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Dubé ID , Arlin ZA, Kalousek DK, Eaves CJ, Eaves AC. Nonclonal hemopoietic progenitor cells detected in long-term marrow cultures from a Turner syndrome mosaic with chronic myeloid leukemia . Blood 1984 ; 64 : 1284 – 1287 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Sharkis SJ , Santos GW, Colvin OM. Elimination of acute myelogenous leukemic cells from marrow and tumor suspension in the rat with 4-hydroperoxycyclophosphamide . Blood 1980 ; 55 : 521 – 523 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Rizzoli V , Mangoni L, Carlo-Stella C. Autologous bone marrow transplantation in acute myelogenous leukemia . Leukemia 1992 ; 6 : 1101 – 1106 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 11 Rizzoli V , Mangoni L, Piovani G, Garau D, Caramatti C, Almici C, Carlo-Stella C. Fractionation of chronic myelogenous leukemia marrow cells by stroma adherence: implications for marrow purging . Leukemia and Lymphoma, 1993 ; (in press). 12 Carlo-Stella C , Mangoni L, Almici C, Garau D, Craviotto L, Piovani G, Caramatti C, Rizzoli V. Differential sensitivity of adherent CFU-Blast, CFU-Mix, BFU-E and CFU-GM to mafosfamide: implications for adjusted dose purging in autologous bone marrow transplantation . Exp Hematol 1992 ; 20 : 328 – 333 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 13 Goldman JM . Bone marrow transplantation for chronic myelogenous leukemia . Curr Opin Oncol 1992 ; 4 : 259 – 263 . Google Scholar Crossref Search ADS PubMed WorldCat 14 Dunbar CE , Stewart FM. Separating the wheat from the chaff: selection of benign hematopoietic cells in chronic myelogenous leukemia . Blood 1992 ; 79 : 1107 – 1110 . Google Scholar Crossref Search ADS PubMed WorldCat 15 Barnett MJ , Eaves AC, Phillips GL. An overview of bone marrow transplantation for chronic myeloid leukemia . Can Med Assoc J 1990 ; 143 : 187 – 193 . Google Scholar OpenURL Placeholder Text WorldCat 16 Carella AM , Gaozza E, Raffo MR, Carlier P, Frassoni F, Valbonesi M, Lercari G, Sessarego M, Defferrari R, Guerrasio A, Saglio G, Canepa L, Gaetani GF, Occhini D. Therapy of acute phase chronic myelogenous leukemia with intensive chemotherapy, blood cell autotransplant and cyclosporine A . Leukemia 1991 ; 5 : 517 – 521 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 17 Carlo-Stella C , Mangoni L, Piovani G, Almici C, Garau D, Caramatti C, Rizzoli V. In vitro marrow purging in chronic myelogenous leukemia: effect of mafosfamide and recombinant granulocyte-macrophage colony-stimulating factor . Bone Marrow Transplant 1991 ; 8 : 265 – 273 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 18 Simonsson B , Öberg G, Björeman M, Björkholm M, Gahrton G, Hast R, Killander A, Turesson I, Udén AM, Vikrot O, Vilén L, Wahlin A, Carneskog J, Westlin J. Intensive treatment in order to minimize the Ph-positive clone in chronic myelogenic leukemia . Leukemia and Lymphoma 1992 ; 7 : 55 – 57 . Google Scholar Crossref Search ADS PubMed WorldCat 19 McGlave PB , Arthur D, Miller WJ, Lasky L, Kersey J. Autologous transplantation for CML using marrow treated ex vivo with human interferon gamma . Bone Marrow Transplant 1990 ; 6 : 115 – 120 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 20 Barnett MJ , Eaves CJ, Phillips GL, Kalousek DK, Klingemann HG, Lansdorp PM, Reece DE, Shepherd JD, Shaw GJ, Eaves AC: Successful autografting in chronic myeloid leukemia after maintenance of marrow in culture . Bone Marrow Transplant 1989 ; 4 : 345 – 351 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 21 Meloni G , Vignetti M, Pinto R, Montefusco E, Simone F, Alimena G. L'autotrapianto nella leucemia mieloide cronica . Prog Ematol Clin 1992 ; 11 : 269 – 273 . Google Scholar OpenURL Placeholder Text WorldCat 22 Reiffers J , Trouette R, Mark G, Montastruc M, Fabères C, Cony-Makhoul P, David B, Bourdeau MJ, Bilhou-Nabera C, Lacombe F, Feuillatre-Fabre F, Vezon G, Bernard Ph, Broustet A. Autologous blood stem cell transplantation for chronic granulocytic leukaemia in transformation: a report of 47 cases . Br J Haematol 1991 ; 77 : 339 – 345 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Brito-Babapulle F , Bowcock SJ, Marcus RE, Apperley J, Th'ng KH, Dowding C, Rassool F, Guo AP, Catovsky D, Galton DAG, McCarthy D, Goldman JM. Autografting for patients with chronic myeloid leukaemia in chronic phase: peripheral blood stem cells may have a finite capacity for maintaining haemopoiesis . Br J Haematol 1989 ; 73 : 76 – 81 . Google Scholar Crossref Search ADS PubMed WorldCat 24 Haines ME , Goldman JM, Worsley AM, McCarthy DM, Wyatt SE, Dowding C, Kearney L, Th'ng KH, Wareheam NJ, Pollock A, Galvin MG, Samson D, Geary G, Catovsky D and Galton DAG. Chemotherapy and autografting for chronic granulocytic leukaemia in transformation: probable prolongation of survival for some patients . Br J Haematol 1984 ; 58 : 711 – 721 . Google Scholar Crossref Search ADS PubMed WorldCat 25 Goldman JM . Autografting for chronic myeloid leukemia . Palliation, cure or nothing? Leukemia and Lymphoma 1992 ; 7 : 51 – 54 . Google Scholar Crossref Search ADS WorldCat 26 Gordon MY , Dowding CR, Riley GP, Goldman JM, Greaves MF. Altered adhesive interactions with marrow stroma of hematopoietic progenitor cells in chronic myeloid leukemia . Nature 1987 ; 328 : 342 – 344 . Google Scholar Crossref Search ADS PubMed WorldCat 27 Gordon MY , Atkinson J, Clarke D, Dowding CR, Goldman JM, Grimsley PG, Siczkowski M, Greaves MF: Deficiency of a phosphatidylinositol-anchored cell adhesion molecule influences hemopoietic progenitor binding to marrow stroma in chronic myeloid leukemia . Leukemia 1991 ; 8 : 693 – 698 . Google Scholar OpenURL Placeholder Text WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Keating, Armand

doi: 10.1002/stem.5530110910pmid: 8298475

Abstract It is now feasible to investigate bcr-abl transcription by the progeny of Ph-positive (Ph+) early and committed hemopoietic progenitor cells from patients with chronic myeloid leukemia (CML). Cells from individual colonies can be bisected and each half analyzed by cytogenetics or the reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect the bcr-abl transcript using internal nested oligonucleotide primers that flank the chimeric gene junction. We previously showed that some Ph+ colonies have undetectable PCR products for bcr-abl. When colonies are generated in the presence of alpha interferon (IFN-α) bcr-abl transcripts are undetectable in the majority of Ph+ colonies. These data suggest a potential mechanism for the action of IFN-α in Ph+ CML and indicate the need for a combined approach with cytogenetics and RT-PCR in analyzing the bcr-abl gene. bcr-abl, CML, PCR References 1 Lugo TG , Witte ON. The BCR-ABL oncogene transforms rat-1 cells and cooperates with v-myc . Mol Cell Biol 1989 ; 9 ( 3 ): 1263 – 1270 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 2 Gishizky ML , Witte ON. Initiation of deregulated growth of multipotent progenitor cells by bcr-abl in vitro . Science 1991 ; 256 : 836 – 839 . Google Scholar Crossref Search ADS WorldCat 3 Daley GQ , Van Etten RA, Baltimore D. Induction of chronic myelogenous leukemia in mice by the P210 bcr-abl gene of the Philadelphia chromosome . Science 1990 ; 247 : 824 – 830 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Konopka JB , Watanabe SM, Witte ON. An alteration of the human c- abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity . Cell 1984 ; 37 : 1035 – 1042 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Konopka JB , Watanabe SM, Singer JW, Collins SJ, Witte ON. Cell lines and clinical isolates derived from Ph1-positive chronic myelogenous leukemia patients express c- abl protein with a common structural alteration . Proc Natl Acad Sci USA 1985 ; 82 : 1810 – 1814 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Keating A , Toor P. In vitro clonal culture of human hematopoietic progenitor cells. In: Walker JM, Pollard JW, eds. Methods in Molecular Biology , Volume 5 . Clifton, New Jersey : Humana Press Inc. , 1990 ; 339 – 346 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 7 Dube ID , Eaves CJ, Kalousek DK, Eaves AC. A method for obtaining high quality chromosome preparations from single hematopoietic colonies on a routine basis . Cancer Genet Cytogenet 1981 ; 4 : 157 – 168 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Kawasaki ES , Clark SS, Coyne MY, Smith SD, Champlin R, Witte ON, McCormick FP. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro . Proc Natl Acad Sci USA 1988 ; 85 : 5698 – 5702 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Keating A , Martin P, Bernstein I, Papayannopoulou T, Raskind W, Singer JW. EM-2 and EM-3: Two new Ph + myeloid cell lines. In: Golde DW, Marks PA, Eds. Normal and Neoplastic Hematopoiesis, UCLA Symposia on Molecular and Cellular Biology , New Series , Vol 9 . New York : Alan R. Liss, Inc. , 1983 ; 513 – 520 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 10 Crump M , Wang X-H, Sermer R, Keating A. Successful pregnancy and delivery during α-interferon therapy for chronic myeloid leukemia . Am J Hematol 1992 ; 40 ( 3 ): 238 – 239 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)