TY - JOUR
AU - Schulze-Osthoff, Klaus
AB - THE Fas/APO-1 receptor is one of the major regulators of apoptosis1á€-7. We report here that Fas/APO-1-mediated apoptosis requires the activation of a new class of cysteine proteases, including interleukin-lβ-converting enzyme (ICE)8á€-10, which are homologous to the product of the Caenorhabditis elegans cell-death gene ced-3 (refs 11, 12). Triggering of Fas/APO-1 rapidly stimulated the proteolytic activity of ICE. Overexpression of ICE, achieved by electroporation and microinjection, strongly potentiated Fas/ APO-1-mediated cell death. In addition, inhibition of ICE activity by protease inhibitors, as well as by transient expression of the pox virus-derived serpin inhibitor CrmA or an antisense ICE construct, substantially suppressed Fas/APO-1-triggered cell death. We conclude that activation of ICE or an ICE-related protease is a critical event in Fas/APO-1-mediated cell death.
TI - Requirement of an ICE/CED-3 protease for Fas/APO-1-mediated apoptosis
JF - Nature
DO - 10.1038/375081a0
DA - 1995-05-04
UR - https://www.deepdyve.com/lp/springer-journals/requirement-of-an-ice-ced-3-protease-for-fas-apo-1-mediated-apoptosis-0eKUqpLLkt
SP - 81
EP - 83
VL - 375
IS - 6526
DP - DeepDyve
ER -