TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on November 7, 2021 - Published by Cold Spring Harbor Laboratory Press ( ) Enhancer control of V D J recombination at the TCRb locus: differential effects on DNA cleavage and joining 1,3 2,3 1 1 William M. Hempel, Patricia Stanhope-Baker, Noe ¨ lle Mathieu, Fang Huang, 2,4 1,4 Mark S. Schlissel, and Pierre Ferrier Centre d’Immunologie Institut National de la Sante ´ et de la Recherche Me ´ dicale–Centre National de la Recherche Scientifique (INSERM-CNRS) de Marseille-Luminy, Marseille Cedex 9, France; Department of Medicine, Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA Deletion of the TCRb transcriptional enhancer (Eb) results in nearly complete inhibition of V(D)J recombination at the TCRb locus and a block in ab T cell development. This result, along with previous work from many laboratories, has led to the hypothesis that transcriptional enhancers affect V(D)J recombination by regulating the accessibility of the locus to the recombinase. Here we test this hypothesis by −/− performing a detailed analysis of the recombination defect in Eb-deleted (Eb ) mice using assays that detect various reaction intermediates and products. We found double-strand DNA breaks at recombination signal 
TI - Enhancer control of<i>V(D)J</i>recombination at the TCRβ locus: differential effects on DNA cleavage and joining
JF - Genes & Development
DO - 10.1101/gad.12.15.2305
DA - 1998-08-01
UR - https://www.deepdyve.com/lp/unpaywall/enhancer-control-of-i-v-d-j-i-recombination-at-the-tcr-locus-20k3cOH5QN
DP - DeepDyve
ER -