TY - JOUR
AU - Douce, Roland
AB - Abstract Isolated spinach chloroplasts purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extrachloroplastic contaminations, and to retain a high rate of CO2-dependent O2 evolution. When suspended in a medium which avoided rupture of the envelope, intact purified chloroplasts progressively lost their phosphate content by passive diffusion. This led to a slow decrease in the uptake of labeled 3-phosphoglyceric acid or orthophosphate (Pi) and in the rate of CO2-dependent O2 evolution by isolated chloroplasts. Under these conditions, there was a good correlation between the rate of CO2-dependent O2 evolution and the concentration of Pi in the stroma space. Addition of Pi to the suspending medium at a final concentration of 10 millimolar, which counterpoised the slow efflux of Pi from the chloroplasts, slowed considerably the decrease in the rate of CO2-dependent O2 evolution. 1 This work was supported in part by grants from the Centre National de la Recherche Scientifique (ERA 847: “Interactions plastescytoplasme-mitochondries”) and the Délégation Générale à la Recherche Scientifique et Technique (contrat MB/P210). This content is only available as a PDF. © 1981 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium 
JF - Plant Physiology
DO - 10.1104/pp.67.3.470
DA - 1981-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/slow-passive-diffusion-of-orthophosphate-between-intact-isolated-3aLi8PTP00
SP - 470
EP - 473
VL - 67
IS - 3
DP - DeepDyve
ER -