TY - JOUR
AU - 
AB - THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 276, No. 17, Issue of April 27, pp. 14075–14082, 2001 © 2001 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Peculiar 2-Aminopurine Fluorescence Monitors the Dynamics of Open Complex Formation by Bacteriophage T7 RNA Polymerase* Received for publication, December 14, 2000 Published, JBC Papers in Press, January 25, 2001, DOI 10.1074/jbc.M011289200 Rajiv P. Bandwar and Smita S. Patel‡ From the Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854 The kinetics of promoter binding and open complex the dynamics of the transcription initiation and elongation mechanisms (1, 2). formation in bacteriophage T7 RNA polymerase was in- vestigated using 2-aminopurine (2-AP) modified promot- The initiation of transcription is a multistep process that ers. 2-AP serves as an ideal probe to measure the kinet- directs the polymerase to the promoter region, where RNA ics of open complex formation because its fluorescence synthesis is initiated. After the RNA polymerase recognizes a is sensitive to both base-unpairing and base-unstacking consensus DNA sequence, the specific binding energy is used to and to the nature of the neighboring bases. All four 2-AP melt a region of the promoter, part of which serves 
TI - Peculiar 2-Aminopurine Fluorescence Monitors the Dynamics of Open Complex Formation by Bacteriophage T7 RNA Polymerase
JF - Journal of Biological Chemistry
DO - 10.1074/jbc.m011289200
DA - 2001-04-01
UR - https://www.deepdyve.com/lp/unpaywall/peculiar-2-aminopurine-fluorescence-monitors-the-dynamics-of-open-4B8nrz79Mw
DP - DeepDyve
ER -