TY - JOUR
AU - Crabtree, Gerald R.
AB - SEQUENCE-SPECIFIC DNA binding activators of gene transcription may be assisted by SWI2(SNF2)
1,2, which contains a DNA-depen-dent ATPase domain3. We have isolated a human complementary DNA encoding a 205K nuclear protein, BRG1, that contains extensive homology to SWI2 and Drosophila brahma
4,5. We report here that a SWI2/BRG1 chimaera with the DNA-dependent ATPase domain replaced by corresponding human sequence restored normal mitotic growth and capacity for transcriptional activation to swi2& minus; yeast cells. Point mutation of the conserved ATP binding site lysine abolished this complementation. This mutation in SW12 exerted a dominant negative effect on transcription in yeast. A lysine to arginine substitution at the corresponding residue of BRG1 also generated a transcriptional dominant negative in human cells. BRG1 is exclusively nuclear and present in a high M
r complex of about 2 & times; 106. These results show that the SWI2 family DNA-dependent ATPase domain has functional con-servation between yeast and humans and suggest that a SWI/SNF protein complex is required for the activation of selective mammalian genes.
TI - BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription
JF - Nature
DO - 10.1038/366170a0
DA - 1993-11-11
UR - https://www.deepdyve.com/lp/springer-journals/brg1-contains-a-conserved-domain-of-the-swi2-snf2-family-necessary-for-4OyWdpIisW
SP - 170
EP - 174
VL - 366
IS - 6451
DP - DeepDyve
ER -