TY - JOUR
AU - Hostettmann, Kurt
AB - Plants represent an extraordinary reservoir of novel molecules and there is currently a resurgence of interest in the vegetable kingdom as a possible source of new lead compounds for introduction into therapeutical screening programs. In order to discover potential new bioactive natural products, the dereplication of crude plant extracts performed prior to isolation work is of crucial importance for avoiding the isolation of a known constituent. In this respect, chemical screening strategies have been developed using hyphenated techniques (LC/UV–DAD, LC–MS and LC–NMR). In our laboratory, these techniques have been fully integrated into the isolation process and are used for the chemical screening of crude plant extracts in complement with on‐line or at‐line bioassays. LC–UV–MS is used as a first dereplication step in combination with UV and MS databases, while LC–NMR is performed in a second step for de novo on‐line structure determination. This approach enables the partial or the complete on‐line identification of natural products in complex matrices such as crude plant extracts. These methods also give a unique possibility to study unstable compounds, which rapidly degrade or which are not separable at a preparative level.
TI - Phytochemistry in the microgram domain—a LC–NMR perspective
JF - Magnetic Resonance in Chemistry
DO - 10.1002/mrc.1631
DA - 2005-01-01
UR - https://www.deepdyve.com/lp/wiley/phytochemistry-in-the-microgram-domain-a-lc-nmr-perspective-50c0Ru0RO0
SP - 697
EP - 709
VL - 43
IS - 9
DP - DeepDyve
ER -