TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on November 4, 2021 - Published by Cold Spring Harbor Laboratory Press A biochemical framework for RNA silencing in plants 1 2 2 1,3 Guiliang Tang, Brenda J. Reinhart, David P. Bartel, and Phillip D. Zamore Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA; Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA RNA silencing phenomena were first discovered in plants, yet only the RNA interference pathway in animals has been subject to biochemical analysis. Here, we extend biochemical analysis to plant RNA silencing. We find that standard wheat germ extract contains Dicer-like enzymes that convert double-stranded RNA (dsRNA) into two classes of small interfering RNAs, as well as an RNA-dependent RNA polymerase activity that can convert exogenous single-stranded RNA into dsRNA. In this plant embryo extract, an endogenous microRNA (miRNA) that lacks perfect complementarity to its RNA targets nonetheless acts as a small interfering RNA. The miRNA guides an endonuclease to cleave efficiently wild-type Arabidopsis PHAVOLUTA mRNA, but not a dominant mutant previously shown to perturb leaf development. This finding supports the view that plant miRNAs direct RNAi and that 
TI - A biochemical framework for RNA silencing in plants
JF - Genes & Development
DO - 10.1101/gad.1048103
DA - 2003-01-01
UR - https://www.deepdyve.com/lp/unpaywall/a-biochemical-framework-for-rna-silencing-in-plants-6R4Eb9klCC
DP - DeepDyve
ER -