TY - JOUR
AU1 - Li, Jin Billy
AU2 - Levanon, Erez Y
AU3 - Yoon, Jung-Ki
AU4 - Aach, John
AU5 - Xie, Bin
AU6 - Leproust, Emily
AU7 - Zhang, Kun
AU8 - Gao, Yuan
AU9 - Church, George M
AB - Adenosine-to-inosine (A-to-I) RNA editing leads to transcriptome diversity and is important for normal brain function. To date, only a handful of functional sites have been identified in mammals. We developed an unbiased assay to screen more than 36,000 computationally predicted nonrepetitive A-to-I sites using massively parallel target capture and DNA sequencing. A comprehensive set of several hundred human RNA editing sites was detected by comparing genomic DNA with RNAs from seven tissues of a single individual. Specificity of our profiling was supported by observations of enrichment with known features of targets of adenosine deaminases acting on RNA (ADAR) and validation by means of capillary sequencing. This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases.
TI - Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing.
JF - Science (New York, N.Y.)
DO - 10.1126/science.1170995
DA - 2009-06-11
UR - https://www.deepdyve.com/lp/pubmed/genome-wide-identification-of-human-rna-editing-sites-by-parallel-dna-6W3Stkc3Af
SP - 1210
EP - 3
VL - 324
IS - 5931
DP - DeepDyve
ER -