TY - JOUR AU - AB - Downloaded from http://mra.asm.org/ on May 13, 2021 by guest crossmark Complete Genome Sequence of NEB 5-alpha, a Derivative of Escherichia coli K-12 DH5 Brian P. Anton, Elisabeth A. Raleigh New England Biolabs, Ipswich, Massachusetts, USA Escherichia coli K-12 DH5 is one of the most popular and widely available laboratory strains, but, surprisingly, no complete genome sequence has been publicly available. Here, we report the complete, finished sequence of NEB 5-alpha (DH5 fhuA2). It should serve as a useful reference for researchers working with DH5. Received 12 September 2016 Accepted 21 September 2016 Published 10 November 2016 Citation Anton BP, Raleigh EA. 2016. Complete genome sequence of NEB 5-alpha, a derivative of Escherichia coli K-12 DH5. Genome Announc 4(6):e01245-16. doi:10.1128/ genomeA.01245-16. Copyright © 2016 Anton and Raleigh. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Brian P. Anton, anton@neb.com. H5, constructed by Douglas Hanahan, is one of the most 97,240 bp in length, extending from mmuP to mhpD, which is con- Dcommonly used Escherichia coli K-12 laboratory strains. It sistent with the original report (8) and contrary to the WGA study (6). is widely commercially available and has TI - Complete Genome Sequence of NEB 5-alpha, a Derivative of Escherichia coli K-12 DH5α JF - Genome Announcements DO - 10.1128/genomea.01245-16 DA - 2016-12-29 UR - https://www.deepdyve.com/lp/unpaywall/complete-genome-sequence-of-neb-5-alpha-a-derivative-of-escherichia-7L3M4Q0008 DP - DeepDyve ER -