TY - JOUR
AU - Luo, Ming-Cheng
AB - Background: Brachypodium distachyon (Brachypodium) has been recognized as a new model species for comparative and functional genomics of cereal and bioenergy crops because it possesses many biological attributes desirable in a model, such as a small genome size, short stature, self-pollinating habit, and short generation cycle. To maximize the utility of Brachypodium as a model for basic and applied research it is necessary to develop genomic resources for it. A BAC-based physical map is one of them. A physical map will facilitate analysis of genome structure, comparative genomics, and assembly of the entire genome sequence. Results: A total of 67,151 Brachypodium BAC clones were fingerprinted with the SNaPshot HICF fingerprinting method and a genome-wide physical map of the Brachypodium genome was constructed. The map consisted of 671 contigs and 2,161 clones remained as singletons. The contigs and singletons spanned 414 Mb. A total of 13,970 gene-related sequences were detected in the BAC end sequences (BES). These gene tags aligned 345 contigs with 336 Mb of rice genome sequence, showing that Brachypodium and rice genomes are generally highly colinear. Divergent regions were mainly in the rice centromeric regions. A dot-plot of Brachypodium contigs against the rice genome sequences revealed remnants 
TI - A BAC-based physical map of Brachypodium distachyon and its comparative analysis with rice and wheat
JF - BMC Genomics
DO - 10.1186/1471-2164-10-496
DA - 2009-10-27
UR - https://www.deepdyve.com/lp/springer-journals/a-bac-based-physical-map-of-brachypodium-distachyon-and-its-7kzLW5hlR0
SP - 1
EP - 13
VL - 10
IS - 1
DP - DeepDyve
ER -