TY - JOUR
AU - Fisher, David E
AB - Our understanding of how chromatin structure influences cellular processes such as transcription and replication has been limited by a lack of nucleosome-positioning data in human cells. We describe a high-resolution microarray approach combined with an analysis algorithm to examine nucleosome positioning in 3,692 promoters within seven human cell lines. Unlike unexpressed genes without transcription-preinitiation complexes at their promoters, expressed genes or genes containing preinitiation complexes exhibit characteristic nucleosome-free regions at their transcription start sites. The combination of these nucleosome data with chromatin immunoprecipitation–chip analyses reveals that the melanocyte master regulator microphthalmia-associated transcription factor (MITF) predominantly binds nucleosome-free regions, supporting the model that nucleosomes limit sequence accessibility. This study presents a global view of human nucleosome positioning and provides a high-throughput tool for analyzing chromatin structure in development and disease.
TI - High-throughput mapping of the chromatin structure of human promoters
JF - Nature Biotechnology
DO - 10.1038/nbt1279
DA - 2007-01-14
UR - https://www.deepdyve.com/lp/springer-journals/high-throughput-mapping-of-the-chromatin-structure-of-human-promoters-7s7Q4UYRDD
SP - 244
EP - 248
VL - 25
IS - 2
DP - DeepDyve
ER -