TY - JOUR
AU - Noble, R L
AB - Excellent results have been obtained for the Fmoc solid-phase syntheses of peptides using the activating reagent 2-(1H-benzotriazol-1-yl)-1,1,3,3,-tetramethyluronium hexafluorophosphate (HBTU). Activation occurs very rapidly in N,N-dimethylformamide and N-methyl-pyrrolidone, optimal solvents for peptide-resin solvation. It has been observed that complete coupling reactions occur in only 10-30 min. Residues such as Arg, Ile, Leu and Val, which often require double coupling by other activation methods, react with high efficiency by single coupling when HBTU is used. The Fmoc/HBTU chemistry has recently been applied to the peptide synthesizers. The incorporation of trityl side-chain protection for Fmoc-Asn and Fmoc-Gln further enhances coupling efficiencies in difficult sequences.
TI - HBTU activation for automated Fmoc solid-phase peptide synthesis.
JF - Peptide research
DA - 1992-06-26
UR - https://www.deepdyve.com/lp/pubmed/hbtu-activation-for-automated-fmoc-solid-phase-peptide-synthesis-82SivfCU05
SP - 95
EP - 101
VL - 4
IS - 2
DP - DeepDyve
ER -