Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.
TI - HiChIP: Efficient and sensitive analysis of protein-directed genome architecture JF - bioRxiv DO - 10.1101/073619 DA - 2016-09-08 UR - https://www.deepdyve.com/lp/biorxiv/hichip-efficient-and-sensitive-analysis-of-protein-directed-genome-8JSJiiaU00 SP - 073619 DP - DeepDyve ER -