TY - JOUR
AU - Li, Chuanfu
AB - Background.This study examined the effect of microRNA-125b (miR-125b) on sepsis-induced cardiac dysfunction.Methods.Mouse hearts were transfected with lentivirus expressing miR-125b (LmiR-125b) 7 days before cecal ligation and puncture (CLP)induced sepsis. Cardiac function was examined by echocardiography before and 6 hours after CLP (n  6/group). Survival was monitored following CLP-induced sepsis (n  12/group).Results.LmiR-125b transfection significantly attenuated cardiac dysfunction due to CLP-induced sepsis. Fractional shortening and ejection fraction values were significantly (P < .05) higher in the LmiR-125btreated CLP group than in the untreated CLP group. Survival outcome in LmiR-125btransfected septic mice was markedly improved, compared with mice with CLP-induced sepsis. Transfection of LmiR-125b into the heart significantly suppressed the expression of ICAM-1 and VCAM-1, decreased the accumulation of macrophages and neutrophils in the myocardium, and decreased serum levels of tumor necrosis factor  and interleukin 1 by targeting tumor necrosis factor receptorassociated factor 6 (TRAF6)mediated nuclear factor B (NF-B) activation. In addition, sepsis-induced myocardial apoptosis was markedly attenuated by LmiR-125b transfection through suppression of p53, Bax, and Bak1 expression. In vitro transfection of endothelial cells with miR-125b mimics attenuate LPS-induced ICAM-1 and VCAM-1 expression by suppressing TRAF6 and NF-B activation.Conclusions.Increased myocardial miR-125b expression attenuates sepsis-induced cardiac dysfunction and improves survival. miR-125b may be a target for septic cardiomyopathy.
TI - MicroRNA-125b Prevents Cardiac Dysfunction in Polymicrobial Sepsis by Targeting TRAF6-Mediated Nuclear Factor B Activation and p53-Mediated Apoptotic Signaling
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/jiw449
DA - 2016-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/microrna-125b-prevents-cardiac-dysfunction-in-polymicrobial-sepsis-by-8k1IPa04dS
SP - 1773
EP - 1783
VL - 214
IS - 11
DP - DeepDyve
ER -