TY - JOUR
AU - Rine, J
AB - In Saccharomyces cerevisiae, the HMR-E silencer blocks site-specific interactions between proteins and their recognition sequences in the vicinity of the silencer. Silencer function is correlated with the firing of an origin of replication at HMR-E. An essential gene with a role in transcriptional silencing was identified by means of a screen for mutations affecting expression of HMR. This gene, known as ORC2, was shown to encode a component of the origin recognition complex that binds yeast origins of replication. A temperature-sensitive mutation in ORC2 disrupted silencing in cells grown at the permissive temperature. At the restrictive temperature, the orc2-1 mutation caused cell cycle arrest at a point in the cell cycle indicative of blocks in DNA replication. The orc2-1 mutation also resulted in the enhanced mitotic loss of a plasmid, suggestive of a defect in replication. These results provide strong evidence for an in vivo role of ORC in both chromosomal replication and silencing, and provide a link between the mechanism of silencing and DNA replication.
TI - Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae.
JF - Science (New York, N.Y.)
DO - 10.1126/science.8266071
DA - 1994-01-21
UR - https://www.deepdyve.com/lp/pubmed/origin-recognition-complex-orc-in-transcriptional-silencing-and-dna-921ziFwxk8
SP - 1838
EP - 44
VL - 262
IS - 5141
DP - DeepDyve
ER -