TY - JOUR
AU - 
AB - Anal Bioanal Chem (2014) 406:1933–1943 DOI 10.1007/s00216-013-7582-x RESEARCH PAPER Aggressive dereplication using UHPLC–DAD–QTOF: screening extracts for up to 3000 fungal secondary metabolites Andreas Klitgaard & Anita Iversen & Mikael R. Andersen & Thomas O. Larsen & Jens Christian Frisvad & Kristian Fog Nielsen Received: 11 September 2013 /Revised: 3 December 2013 /Accepted: 14 December 2013 /Published online: 18 January 2014 The Author(s) 2014. This article is published with open access at Springerlink.com Abstract In natural-product drug discovery, finding new was investigated on reference standards, and the overall meth- compounds is the main task, and thus fast dereplication of od was used on extracts of Aspergillus carbonarius and Pen- known compounds is essential. This is usually performed by icillium melanoconidium, revealing new nitrogen-containing manual liquid chromatography-ultraviolet (LC-UV) or visible biomarkers for both species. light-mass spectroscopy (Vis-MS) interpretation of detected . . . . peaks, often assisted by automated identification of previously Keywords Metabolomics Mycotoxin NRPS LC–MS . . identified compounds. We used a 15 min high-performance UPLC Polyketide Nonribosomal peptide liquid chromatography–diode array detection (UHPLC– DAD)–high-resolution MS method (electrospray ionization + − (ESI) or ESI ), followed by 10–60 s of automated data Introduction analysis for up to 3000 relevant 
TI - Aggressive dereplication using UHPLC–DAD–QTOF: screening extracts for up to 3000 fungal secondary metabolites
JF - Analytical and Bioanalytical Chemistry
DO - 10.1007/s00216-013-7582-x
DA - 2014-01-18
UR - https://www.deepdyve.com/lp/unpaywall/aggressive-dereplication-using-uhplc-dad-qtof-screening-extracts-for-9PwLnTVL3n
DP - DeepDyve
ER -