TY - JOUR
AU1 - Gombert, Wendy M.
AU2 - Farris, Stephen D.
AU3 - Rubio, Eric D.
AU4 - Morey-Rosler, Kristin M.
AU5 - Schubach, William H.
AU6 - Krumm, Anton
AB - Insulator elements and matrix attachment regions are essential for the organization of genetic information within the nucleus. By comparing the pattern of histone modifications at the mouse and human c- myc alleles, we identified an evolutionarily conserved boundary at which the c- myc transcription unit is separated from the flanking condensed chromatin enriched in lysine 9-methylated histone H3. This region harbors the c -m yc insulator e lement (MINE), which contains at least two physically separable, functional activities: enhancer-blocking activity and barrier activity. The enhancer-blocking activity is mediated by CTCF. Chromatin immunoprecipitation assays demonstrate that CTCF is constitutively bound at the insulator and at the promoter region independent of the transcriptional status of c- myc . This result supports an architectural role of CTCF rather than a regulatory role in transcription. An additional higher-order nuclear organization of the c- myc locus is provided by matrix attachment regions (MARs) that define a domain larger than 160 kb. The MARs of the c- myc domain do not act to prevent the association of flanking regions with lysine 9-methylated histones, suggesting that they do not function as barrier elements.
TI - The c-myc Insulator Element and Matrix Attachment Regions Define the c-myc Chromosomal Domain
JF - Molecular and Cellular Biology
DO - 10.1128/MCB.23.24.9338-9348.2003
DA - 2003-12-15
UR - https://www.deepdyve.com/lp/american-society-for-microbiology/the-c-myc-insulator-element-and-matrix-attachment-regions-define-the-c-9vvi3SGRMj
VL - 23
IS - 24
DP - DeepDyve
ER -