TY - JOUR AU - Daubenmire, Rexford F. AB - Ecology, Vol. 21, No. 4 514 NOTES AND COMMENT 1. Small lumps of the originally moist peat vascular cryptogams after 3-5 chlorinations are treated with hydrofluoric acid in wax con­ and extractions with hot sodium sulphite. On tainers until freed from sand, diatoms, and the contrary, the dark, more amorphous, lowest sponge spicules. statum of the peat requires from 8-10 chlorina­ 2. The lumps are thoroughly washed for a tions and extractions for the removal of the number of hours in gently circulating water. dark-colored humic substances. In other words, 3. The slightly alkaline tap water is poured the number of chlorinations and of extractions off and replaced by saturated chlorine water. with sodium sulphite should be varied according At this stage, the lumps of desilicified peat to the composition of the material that is being readily disintegrate upon shaking (stirring with studied. a glass rod if necessary) into more or less The pretreatment with hydrofluoric acid plays minute fragments. The chlorine is allowed to no vitally significant role, other than that of re­ act for from 2-5 minutes. The preparation is moving inorganic constituents. This may be then centrifuged and the liquid drained off. demonstrated by applying the TI - Exclusure Technique in Ecology JF - Ecology DO - 10.2307/1930293 DA - 1940-10-01 UR - https://www.deepdyve.com/lp/wiley/exclusure-technique-in-ecology-A5JC9rAPaA SP - 514 EP - 515 VL - 21 IS - 4 DP - DeepDyve ER -