TY - JOUR
AU - 
AB - THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 274, No. 26, Issue of June 25, pp. 18741–18746, 1999 © 1999 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Identification of the Plasma Membrane H -Biotin Symporter of Saccharomyces cerevisiae by Rescue of a Fatty Acid-auxotrophic Mutant* (Received for publication, March 22, 1999, and in revised form, April 14, 1999) Ju ¨ rgen Stolz‡, Ursula Hoja§, Sandra Meier§, Norbert Sauer‡¶, and Eckhart Schweizer§ From the ‡Lehrstuhl Botanik II, Molekulare Pflanzenphysiologie, and the §Lehrstuhl fu ¨ r Biochemie, Universita ¨t Erlangen-Nu ¨ rnberg, Staudtstrasse 5, D-91058 Erlangen, Germany Bakers’ yeast is auxotrophic for biotin (vitamin H) and thesis, i.e. the carboxylation of acetyl-CoA to malonyl-CoA. A depends on the efficient uptake of this compound from loss of functionally active Acc1p produces a lethal phenotype the environment. A mutant strain with strongly reduced that cannot be rescued by the supply of fatty acids from the biotin uptake and with reduced levels of protein bioti- growth medium (8, 9). This is caused by an impairment of the nylation was identified. The strain was auxotrophic for malonyl-CoA-dependent elongation of long-chain fatty acids to long-chain fatty acids, and this auxotrophy could be 
TI - Identification of the Plasma Membrane H+-Biotin Symporter of Saccharomyces cerevisiae by Rescue of a Fatty Acid-auxotrophic Mutant
JF - Journal of Biological Chemistry
DO - 10.1074/jbc.274.26.18741
DA - 1999-06-01
UR - https://www.deepdyve.com/lp/unpaywall/identification-of-the-plasma-membrane-h-biotin-symporter-of-B7WUmtZuJT
DP - DeepDyve
ER -