TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on October 24, 2021 - Published by Cold Spring Harbor Laboratory Press The functional BPV-1 E2 trans-activating rotem can act as a repressor y preventing formation of the initiation complex Nathalie Dostatni, 1 Paul F. Lambert, 2'3 Rosemary Sousa, a Jonathan Ham, 1 Peter M. Howley, 2 and Moshe Yaniv I ~Unit4 des Virus Oncoghnes, D4partement des Biotechnologies, UA 1149 du Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris, France; 2Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892 USA The products encoded by the E2 open reading frame of the papillomaviruses are DNA-binding transcription factors involved in the positive or negative regulation of multiple viral promoters. To further understand the mechanisms by which the same transcription factor may act differentially, the full-length BPV-1 E2 protein was expressed and purified from yeast and assayed in vitro for its capacity to modulate transcription. E2 stimulated transcription of the HSV thymidine kinase (TK) promoter when E2-binding sites were positioned in an enhancer configuration -100 bp upstream of the promoter start site. In contrast, the same full-length E2 protein repressed transcription of the HPV-18 E6/E7 Plos promoter. This repression was mediated through binding to the 
TI - The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex.
JF - Genes & Development
DO - 10.1101/gad.5.9.1657
DA - 1991-09-01
UR - https://www.deepdyve.com/lp/unpaywall/the-functional-bpv-1-e2-trans-activating-protein-can-act-as-a-BXvcq9aLG7
DP - DeepDyve
ER -