TY - JOUR
AU - Rouquette, J.
AB - INTRODUCTIONHuman skin is a large opaque organ covering the body that provides an efficient barrier to the external environment. It has 3D organization and is composed by the superposition of three layers: a stratified cellular epidermis, a dermis made of connective tissue, and a subcutaneous tissue composed of fat cells. Skin appendages are organized in a perpendicular plane to these layers, originating from the dermis or the hypodermis and connecting to the epidermis. These include sweat glands, sebaceous glands and hair follicles. An erector pili muscle is also attached to several hair follicles and is responsible for hair erection. Finally, the skin is also connected by nervous, vascular and lymphatic systems also organized in a perpendicular plane to the skin surface.Histological examination of human skin is the gold standard approach to analyse the morphology of this tissue. Nevertheless, this method only allows 2D observation of thin tissue sections with a microscope, making 3D structure extrapolation very difficult. The technique of high‐resolution episcopic microscopy (HREM) enables the combination of histology and digital volume to study the 3D structure. Alternatively, several technologies are used to perform high‐resolution imaging of whole tissue, such as confocal and two‐photon microscopies. The main advantage of 
TI - 3D imaging of cleared human skin biopsies using light‐sheet microscopy: A new way to visualize in‐depth skin structure
JF - Skin Research and Technology
DO - 10.1111/srt.12429
DA - 2018-01-01
UR - https://www.deepdyve.com/lp/wiley/3d-imaging-of-cleared-human-skin-biopsies-using-light-sheet-microscopy-BaujlqR374
SP - 294
EP - 303
VL - 24
IS - 2
DP - DeepDyve
ER -