TY - JOUR
AU - Booth, B. C.
AB -  Abstract A simple cryo-preservation method of preparing phytoplankton samples for epifluorescence microscopy allowed observation of autofluorescence of 52 species of nano- and picoplankton after storage periods of 6 months and longer. Samples were fixed in glutaraldehyde, concentrated at sea using filtration, and stored in oil at --15 °C. Using this method cell counts of field populations of Synechococcus spp. and larger phototrophs made after 2 years of storage were not significantly different from counts made on the day of collection. Most of the species of nanoplankton tested were destroyed by fixation in formalin. Lugol's fixative was the gentlest preservative tested but inappropriate for epifluorescence microscopy. Glutaraldehyde caused shrinkage of cell diameters of ca 13%, and when combined with critical point drying shrinkage of ca 40%. Introduction Many studies now point to the general importance in all oceans of phytoplankton in the pico- and nanoplankton size classes (e.g. Anderson 1965, Booth et al. 1982, Furuya and Marumo 1983, Murphy and Haugen 1985, Davis et al. 1985). Many of these organisms are delicate and require special preservation techniques and methods of analysis different from those traditionally employed for larger, net phytoplankton. Epifluorescence microscopy (EFM) is used to enumerate and size 
TI - The Use of Autofluorescence for Analyzing Oceanic Phytoplankton Communities
JF - Botanica Marina
DO - 10.1515/botm.1987.30.2.101
DA - 1987-01-01
UR - https://www.deepdyve.com/lp/de-gruyter/the-use-of-autofluorescence-for-analyzing-oceanic-phytoplankton-BuA9VKSQQx
SP - 101
EP - 108
VL - 30
IS - 2
DP - DeepDyve
ER -