TY - JOUR
AU - Arruda, Paulo
AB - The Arabidopsis thaliana uncoupling protein (UCP) gene was expressed in Escherichia coli and isolated protein reconstituted into liposomes. Linoleic acid‐induced H+ fluxes were sensitive to purine nucleotide inhibition with an apparent K
i (in mM) of 0.8 (GDP), 0.85 (ATP), 0.98 (GTP), and 1.41 (ADP); the inhibition was pH‐dependent. Kinetics of AtPUMP1‐mediated H+ fluxes were determined for lauric, myristic, palmitic, oleic, linoleic, and linolenic acids. Properties of recombinant AtPUMP1 indicate that it represents a plant counterpart of animal UCP2 or UCP3. This work brings the functional and genetic approaches together for the first time, providing strong support that AtPUMP1 is truly an UCP.
TI - Functional reconstitution of Arabidopsis thaliana plant uncoupling mitochondrial protein (AtPUMP1) expressed in Escherichia coli
JF - Febs Letters
DO - 10.1016/S0014-5793(01)02835-6
DA - 2001-09-14
UR - https://www.deepdyve.com/lp/wiley/functional-reconstitution-of-arabidopsis-thaliana-plant-uncoupling-C1kxjKNiRC
SP - 240
EP - 244
VL - 505
IS - 2
DP - DeepDyve
ER -