TY - JOUR
AU - Duine,, J.A.
AB - Summary When grown on glucose in K+-limited chemostat culture, or in batch culture with or without 2,4-dinitrophenol, several strains of Escherichia coli (including the type strain) were found to synthesize a quinoprotein glucose dehydrogenase apoenzyme. The pyridine nucleotides, NAD+ and NADP+, would not serve as cofactor, but activity could be demonstrated upon addition of 2,7,9-tricarboxy-1 H-pyrrolo(2,3-f)quinoline-4,5-dione (PQQ). Thus, in the presence of PQQ, but not in its absence, glucose was oxidized to gluconic acid. A mutant of E. coli PC 1000 was isolated that lacked Enzyme I of the phosphoenolpyruvate phosphotransferase system (PTS) but still synthesized the glucose dehydrogenase apoenzyme. Whereas this mutant would not grow on glucose in the absence of PQQ, it would do so in the presence of low concentrations (1 µM) of this cofactor. On the basis of these observations, it is concluded that the protein (apoenzyme) formed is a genuine glucose dehydrogenase, but that it is not functional in growing cells due to their inability to synthesize the appropriate cofactor (PQQ), at least under these conditions. Glucose dehydrogenase, pyrrolo-quinoline quinone, Escherichia coli References [1] O'Brien R.W. Neijssel O.M. Tempest D.W. ( 1980 ) J. Gen. Microbiol. 116 , 305 – 314 . PubMed [2] Neijssel O.M. Tempest D.W. ( 1975 ) Arch. Microbiol. 105 , 183 – 185 . Crossref Search ADS PubMed [3] Neijssel O.M. ( 1977 ) FEMS Microbiol. Lett. 1 , 47 – 50 . Crossref Search ADS [4] Neijssel O.M. Tempest D.W. Postma P.W. Duine J.A. Frank Jzn J. ( 1983 ) FEMS Microbiol. Lett. 20 , 35 – 39 . Crossref Search ADS [5] Neijssel O.M. Hardy G.P.M.A. Lansbergen J.C. Tempest D.W. O'Brien R.W. ( 1980 ) Arch. Microbiol. 125 , 175 – 179 . Crossref Search ADS PubMed [6] Evans C.G.T. Herbert D. Tempest D.W. ( 1970 ) in Methods in Microbiology ( Norris J.R. Ribbons D.W., Eds.) Vol. 2 , pp. 275 – 327 . Academic Press , London . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [7] Herbert D. Phipps P.J. Tempest D.W. ( 1965 ) Lab. Pract. 14 , 1150 – 1161 . PubMed [8] Duine J.A. Frank Jzn J. van Zeeland J.K. ( 1979 ) FEBS Lett. 108 , 443 – 446 . Crossref Search ADS PubMed [9] Herbert D. Phipps P.J. Strange R.E. ( 1971 ) in Methods in Microbiology ( Norris J.R. Ribbons D.W., Eds.), Vol. 5B , pp. 209 – 344 . Academic Press , London . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [10] Möllering H. Bergmeyer H.U. ( 1974 ) in Methoden der Enzymatischen Analyse , 3rd ed., Vol. 2 , pp. 1288 – 1292 . Verlag Chemie , Weinheim . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [11] Scholte B.J. Schuitema A.R.J. Postma P.W. ( 1982 ) J. Bacteriol. 149 , 576 – 586 . PubMed [12] Michaelis L. Granick S. ( 1943 ) J. Am. Chem. Soc. 65 , 1747 – 1755 . Crossref Search ADS [13] Corey E.J. Tramontano A. ( 1981 ) J. Am. Chem. Soc. 103 , 5599 – 5600 . Crossref Search ADS [14] Van Schie B.J. Van Dijken J.P. Kuenen J.G. ( 1984 ) FEMS Microbiol. Lett. , in press . This content is only available as a PDF. © 1984 Federation of European Microbiological Societies
TI - Evidence of a quinoprotein glucose dehydrogenase apoenzyme in several strains of Escherichia coli
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1984.tb01329.x
DA - 1984-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/evidence-of-a-quinoprotein-glucose-dehydrogenase-apoenzyme-in-several-C51Eimv3AZ
SP - 329
EP - 333
VL - 24
IS - 2-3
DP - DeepDyve
ER -