TY - JOUR
AU - Mathies, Richard A.
AB - © 1996 Nature Publishing Group http://www.nature.com/naturemedicine IMAGE UNAVAILABLE FOR COPYRIGHT REASONS NEW TEC:HNOI. OGV Energy transfer primers: A new fluorescence labeling paradigm for DNA sequencing and analysis 2 1 }INGYUE Ju 1, ALEXANDER N. GLAZER & RICHARD A. MATHIES 'Departments of Chemistry and zMolecular and Cell Biology, University of California, Berkeley, California 94720, USA Correspondence should be addressed to R.A.M. The ability to sequence DNA accurately addressed by examining the properties phoretic mobility shift of the DNA and rapidly is revolutionizing biology demanded of an "ideal" set of four differ­ sequencing fragments. The spectroscopic and medicine. The confluence of the ent fluorophores for DNA sequencing. criteria cannot be met optimally by the massive Human Genome Project, the These dyes should have a similar high properties of single fluorescent dye sequencing of other bacterial and eukary­ molar absorbance at a common excita­ molecules, and indeed are poorly satisfied otic genomes, and the explosive growth tion wavelength, exhibit strong and by currently used sets of fluorescent tags. of DNA sequence-based diagnostics is well-separated fluorescence emissions By exploiting the concept of resonance driving an increasing need for high­ with high fluorescence quantum yields, fluorescence energy transfer (ET), we throughput sequencing. This need 
TI - Energy transfer primers: A new fluorescence labeling paradigm for DNA sequencing and analysis
JF - Nature Medicine
DO - 10.1038/nm0296-246
DA - 1996-02-01
UR - https://www.deepdyve.com/lp/springer-journals/energy-transfer-primers-a-new-fluorescence-labeling-paradigm-for-dna-CMS6K2yuqJ
SP - 246
EP - 249
VL - 2
IS - 2
DP - DeepDyve
ER -