TY - JOUR
AU - Mohan,  M.
AB - 122 91 91 1 1 S. Nair J. S. Bentur U. Prasada Rao M. Mohan NII Campus International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg 110067 New Delhi India Directorate of Rice Research 500 030 Rajendranagar, Hyderabad India Abstract We have developed a polymerase chain reaction (PCR)-based assay that could effectively reduce the time period required to screen and select for Gall Midgeresistant rice lines under field conditions. The primers for the assay were designed on the basis of sequence information of two phenotype specific random amplified polymorphic DNA fragments which were found to be tightly linked to Gall Midge biotype-1 resistance gene ( Gm2 ). The two RAPD fragments, F8 1700 in the susceptible parent ‘ARC6650’ and F10 600 in the resistant parent ‘Phalguna’, were identified after screening 5450 loci using 520 random primers on genomic DNAs of ‘ARC6650’ and ‘Phalguna’. These primers, when used in a multiplexed PCR, amplified specifically a 1.7-kb and 0.6-kb fragment in the susceptible and resistant parents, respectively. When this assay was performed on genomic DNAs of 44 recombinant inbred lines derived from ‘ARC6650’ x ‘Phalguna’ and 5 lines derived from other crosses where one of the parents was ‘Phalguna’, ‘ARC6650’ or their derivatives, the primers amplified a 1.7-kb fragment in all of the susceptible lines or a 0.6-kb fragment in all of the resistant ones. These markers can be of potential use in the marker-aided selection of Gall Midge biotype-1 resistant phenotypes. As screening for resistance can now be conducted independent of the availability of insects, the breeding of resistant varieties can be hastened.
TI - DNA markers tightly linked to a gall midge resistance gene ( Gm2 ) are potentially useful for marker-aided selection in rice breeding
JF - TAG Theoretical and Applied Genetics
DO - 10.1007/BF00220860
DA - 1995-07-01
UR - https://www.deepdyve.com/lp/springer-journals/dna-markers-tightly-linked-to-a-gall-midge-resistance-gene-gm2-are-CR7Bn51LUJ
SP - 68
EP - 73
VL - 91
IS - 1
DP - DeepDyve
ER -