TY - JOUR
AU - Ohlsson, Rolf
AB - Chromatin insulators demarcate expression domains by blocking the cis effects of enhancers or silencers in a position-dependent manner
1,2
. We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation. Chromatin immunoprecipitation analysis showed that a poly(ADP-ribosyl)ation mark, which exclusively segregates with the maternal allele of the insulator domain in the H19 imprinting control region, requires the bases that are essential for interaction with CTCF
3
. Chromatin immunoprecipitation–on–chip analysis documented that the link between CTCF and poly(ADP-ribosyl)ation extended to more than 140 mouse CTCF target sites. An insulator trap assay showed that the insulator function of most of these CTCF target sites is sensitive to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase activity. We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions.
TI - Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
JF - Nature Genetics
DO - 10.1038/ng1426
DA - 2004-09-07
UR - https://www.deepdyve.com/lp/springer-journals/poly-adp-ribosyl-ation-regulates-ctcf-dependent-chromatin-insulation-CSO48HMAjk
SP - 1105
EP - 1110
VL - 36
IS - 10
DP - DeepDyve
ER -