TY - JOUR
AU - Sears, L E
AB - A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.
TI - Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing.
JF - BioTechniques
DA - 1992-01-02
UR - https://www.deepdyve.com/lp/pubmed/application-of-a-novel-chemiluminescence-based-dna-detection-method-to-DtktPnzH8D
SP - 102, 106, 108
EP - 4, 106, 9
VL - 11
IS - 1
DP - DeepDyve
ER -