TY - JOUR
AU - Mazzolini, L.
AB - Abstract The changes in gene expression associated with the reinitiation of cell division and subsequent progression through the cell cycle in Arabidopsis thaliana cell-suspension cultures were investigated. Partial synchronization of cells was achieved by a technique combining phosphate starvation and a transient treatment with the DNA replication inhibitor aphidicolin. Six cDNAs corresponding to genes highly induced in proliferating cells and showing cell-cycle-regulated expression were obtained by the mRNA differential display technique. Full-length cDNA clones (cH2BAt and cH2AvAt) corresponding to two of the display products were subsequently isolated. The cH2BAt clone codes for a novel histone H2B protein, whereas the cH2AvAt cDNA corresponds to a gene encoding a new member of the highly conserved histone H2A.F/Z subfamily of chromosomal proteins. Further studies indicated that H2AvAt mRNA expression is tightly correlated with cell proliferation in cell-suspension cultures, and that closely related analogs of the encoded protein exist in Arabidopsis. The implications of the conservation of histone H2A.F/Z variants in plants are discussed. This content is only available as a PDF. Copyright © 1997 by American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Identification of Proliferation-Induced Genes in Arabidopsis thaliana (Characterization of a New Member of the Highly Evolutionarily Conserved Histone H2A.F/Z Variant Subfamily)
JF - Plant Physiology
DO - 10.1104/pp.115.4.1385
DA - 1997-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/identification-of-proliferation-induced-genes-in-arabidopsis-thaliana-EaiWKXLBFO
SP - 1385
EP - 1395
VL - 115
IS - 4
DP - DeepDyve
ER -