TY - JOUR
AU1 - Ong, Shao-En
AU2 - Blagoev, Blagoy
AU3 - Kratchmarova, Irina
AU4 - Kristensen, Dan Bach
AU5 - Steen, Hanno
AU6 - Pandey, Akhilesh
AU7 - Mann, Matthias
AU8 - 
AB - Research Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics* Shao-En Ong‡, Blagoy Blagoev‡, Irina Kratchmarova‡, Dan Bach Kristensen§, Hanno Steen‡¶, Akhilesh Pandey‡
, and Matthias Mann‡** Quantitative proteomics has traditionally been performed 25 years ago (1, 2). In 2D gel electrophoresis, quantitation is by two-dimensional gel electrophoresis, but recently, achieved by recording differences in the staining pattern of mass spectrometric methods based on stable isotope proteins derived from two states of cell populations or tissues. quantitation have shown great promise for the simultane- Therefore, in addition to obtaining increasingly higher resolu- ous and automated identification and quantitation of tion, technology improvements in the 2D gel community have complex protein mixtures. Here we describe a method, been directed toward the image analysis of 2D gels and the termed SILAC, for stable isotope labeling by amino acids relative quantitation of protein spots by their intensity of stain- in cell culture, for the in vivo incorporation of specific ing (3– 6). amino acids into all mammalian proteins. Mammalian cell Mass spectrometry has long been used in a quantitative lines are grown in media lacking a standard essential amino acid but supplemented with 
TI - Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics
JF - Molecular & Cellular Proteomics
DO - 10.1074/mcp.m200025-mcp200
DA - 2002-05-01
UR - https://www.deepdyve.com/lp/unpaywall/stable-isotope-labeling-by-amino-acids-in-cell-culture-silac-as-a-FGgeCW2sEQ
DP - DeepDyve
ER -