TY - JOUR AU - Clark, Susan J. AB - BioTechniques ISSN: 0736-6205 (Print) 1940-9818 (Online) Journal homepage: www.tandfonline.com/journals/ibtn20 Cytosine Methylation: Quantitation by Automated TM Genomic Sequencing and GENESCAN Analysis Cheryl L. Paul & Susan J. Clark To cite this article: Cheryl L. Paul & Susan J. Clark (1996) Cytosine Methylation: Quantitation TM by Automated Genomic Sequencing and GENESCAN Analysis, BioTechniques, 21:1, 126-133, DOI: 10.2144/96211rr04 To link to this article: https://doi.org/10.2144/96211rr04 © 2018 Author(s) Published online: 02 Aug 2018. Submit your article to this journal Article views: 303 View related articles Citing articles: 2 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=ibtn20 Research Reports Cytosine Methylation: Quantitation by Automated Genomic Sequencing and GENESCAN™ ™ Analysis BioTechniques 21:126-133 (July 1996) INTRODUCTION cation of methylation, we have found Cheryl L. Paul and Susan J. 1,2 accurate quantitation to be difficult. For Clark example, densitometric scanning of ra- Kanematsu Laboratories, Royal Bisulfite genomic sequencing pro- diolabeled sequencing gels can be un- Prince Alfred Hospital, Camper- vides a positive and efficient method reliable due to high background bands down, and CSIRO, Division of for detecting individual 5-methylcyto- often observed on direct PCR sequenc- Biomolecular Engineering, sine (5-MeC) residues in genomic ing gels, whereas automated direct TI - Cytosine Methylation: Quantitation by Automated Genomic Sequencing and GENESCAN TM Analysis JF - BioTechniques DO - 10.2144/96211rr04 DA - 1996-07-01 UR - https://www.deepdyve.com/lp/unpaywall/cytosine-methylation-quantitation-by-automated-genomic-sequencing-and-GbA3msjN9F DP - DeepDyve ER -