TY - JOUR
AU - Wisman,  E.
AB - The development of an Arabidopsis population via single-seed descent is described which includes 3,000 lines that carry approximately 15,000 independent insertions of the autonomous maize element En-1 . A PCR strategy is outlined which allows the recovery of En-1- insertion mutants among this population in any random gene sequence of Arabidopsis thaliana . The method employs PCR reactions on pooled DNA. Positive amplification using a target-specific primer and an En-1 -specific primer on row, column and single-tray pools identifies the putative insertion mutant. In a control experiment two insertion mutants of the PIN gene were successfully identified. In addition, a new independent insertion in the PIN gene was detected which was transmitted to the next generation and showed co-segregation with the pin phenotype. These examples demonstrate that the inheritance of inserts of the autonomous element En-1 is stable enough to make a proper genetic analysis feasible in a genomic background with multiple En-1 inserts.
TI - Successful PCR-based reverse genetic screens using an En-1-mutagenised Arabidopsis thaliana population generated via single-seed descent
JF - TAG Theoretical and Applied Genetics
DO - 10.1007/s001220050949
DA - 1998-10-01
UR - https://www.deepdyve.com/lp/springer-journals/successful-pcr-based-reverse-genetic-screens-using-an-en-1-mutagenised-HDk0ljQPna
SP - 729
EP - 734
VL - 97
IS - 5
DP - DeepDyve
ER -