TY - JOUR
AU - Mandrup, Susanne
AB - Background: The transcription factors peroxisome proliferator activated receptor g (PPARg) and CCAAT/enhancer binding protein a (C/EBPa) are key transcriptional regulators of adipocyte differentiation and function. We and others have previously shown that binding sites of these two transcription factors show a high degree of overlap and are associated with the majority of genes upregulated during differentiation of murine 3T3-L1 adipocytes. Results: Here we have mapped all binding sites of C/EBPa and PPARg in human SGBS adipocytes and compared these with the genome-wide profiles from mouse adipocytes to systematically investigate what biological features correlate with retention of sites in orthologous regions between mouse and human. Despite a limited interspecies retention of binding sites, several biological features make sites more likely to be retained. First, co-binding of PPARg and C/EBPa in mouse is the most powerful predictor of retention of the corresponding binding sites in human. Second, vicinity to genes highly upregulated during adipogenesis significantly increases retention. Third, the presence of C/EBPa consensus sites correlate with retention of both factors, indicating that C/EBPa facilitates recruitment of PPARg. Fourth, retention correlates with overall sequence conservation within the binding regions independent of C/EBPa and PPARg sequence patterns, indicating that other transcription factors work 
TI - Cross species comparison of C/EBPα and PPARγ profiles in mouse and human adipocytes reveals interdependent retention of binding sites
JF - BMC Genomics
DO - 10.1186/1471-2164-12-152
DA - 2011-03-16
UR - https://www.deepdyve.com/lp/springer-journals/cross-species-comparison-of-c-ebp-and-ppar-profiles-in-mouse-and-human-HZXHv470t0
SP - 1
EP - 16
VL - 12
IS - 1
DP - DeepDyve
ER -