TY - JOUR AU1 - Yao, Hongni AU2 - Xie, Quan AU3 - Xue, Shulin AU4 - Luo, Jing AU5 - Lu, Jikang AU6 - Kong, Zhongxin AU7 - Wang, Yongpan AU8 - Zhai, Wenling AU9 - Lu, Nan AU1 - Wei, Rong AU1 - Yang, Yang AU1 - Han, Yuzhou AU1 - Zhang, Yong AU1 - Jia, Haiyan AU1 - Ma, Zhengqiang AB - Key message A major QTL QSpl.nau-7D, named HL2, was validated for its ee ff cts on head length and kernel number per spike using NIL, and mapped to a 0.2 cM interval using recombinants. Abstract Improvement in wheat inflorescence traits such as spike or head length and spikelet number provides an important avenue to increase grain yield potential. In a previous study, QSpl.nau-7D, the major QTL for head length on chromosome 7D, was identified in the recombinant inbred lines derived from Nanda2419 and Wangshuibai. To validate and precisely map this QTL, the Wangshuibai allele was transferred to elite cultivar Yangmai15 through marker-assisted selection. Com- pared with the recurrent parent, the resultant near-isogenic line (NIL) yielded not only 28% longer spikes on the average but also more spikelets and kernels per spike. Moreover, the NIL had a lower spikelet density and did not show significant kernel weight change. In the F population derived from the NIL, QSpl.nau-7D acted like a single semi-dominant gene controlling head length and was therefore designated as Head Length 2 (HL2). With this population, a high-density genetic map was constructed mainly using newly developed markers, and 100 homozygous recombinants including 17 genotypes were obtained. Field experiments showed TI - HL2 on chromosome 7D of wheat (Triticum aestivum L.) regulates both head length and spikelet number JF - TAG Theoretical and Applied Genetics DO - 10.1007/s00122-019-03315-2 DA - 2019-02-27 UR - https://www.deepdyve.com/lp/springer-journals/hl2-on-chromosome-7d-of-wheat-triticum-aestivum-l-regulates-both-head-J2xOsNv360 SP - 1789 EP - 1797 VL - 132 IS - 6 DP - DeepDyve ER -