TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on October 11, 2021 - Published by Cold Spring Harbor Laboratory Press Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition 1 2 Krishnan Venkataraman, Kirk M. Brown, and Gregory M. Gilmartin Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA At least half of all human pre-mRNAs are subject to alternative 3
 processing that may modulate both the coding capacity of the message and the array of post-transcriptional regulatory elements embedded within the 3
 UTR. Vertebrate poly(A) site selection appears to rely primarily on the binding of CPSF to an A(A/U)UAAA hexamer upstream of the cleavage site and CstF to a downstream GU-rich element. At least one-quarter of all human poly(A) sites, however, lack the A(A/U)UAAA motif. We report that sequence-specific RNA binding of the human 3
 processing factor CFI can function as a primary determinant of poly(A) site recognition in the absence of the A(A/U)UAAA motif. CFI is sufficient to direct sequence-specific, A(A/U)UAAA-independent poly(A) addition in vitro through the recruitment of the CPSF subunit hFip1 and poly(A) polymerase to the RNA substrate. ChIP analysis indicates that CFI is recruited to the transcription unit, along 
TI - Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition
JF - Genes & Development
DO - 10.1101/gad.1298605
DA - 2005-06-01
UR - https://www.deepdyve.com/lp/unpaywall/analysis-of-a-noncanonical-poly-a-site-reveals-a-tripartite-mechanism-JfyEiZN0G6
DP - DeepDyve
ER -