TY - JOUR
AU - Ordovas, Jose M.
AB - [Quantification of the level of expression for a specific gene in samples obtained during various experimental conditions is becoming increasingly important as attempts are made to gain knowledge relating to how these altered conditions affect cells at the molecular level. Traditionally, an estimate of the copy number of a gene transcription product in a sample was made by employing traditional Northern blot, dot-blot hybridization, or solution hybridization techniques. These techniques are labor-intensive and usually require microgram quantities of total RNA in order to detect specific mRNAs. The low sensitivity of these assays makes it difficult to estimate differences in expression less than fourfold. In addition, genes with low levels of expression often cannot be detected at all by these assays.]
TI - Lipoprotein Protocols: Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method
DA - 1998-01-01
UR - https://www.deepdyve.com/lp/springer-journals/lipoprotein-protocols-quantification-of-mrna-by-polymerase-chain-L7kXRtKH3v
DP - DeepDyve
ER -