TY - JOUR
AU1 - Cséke, Csaba
AU2 - Stitt, Mark
AU3 - Balogh, Árpád
AU4 - Buchanan, Bob B.
AB - An enzyme catalyzing the hydrolytic conversion of fructose 2,6‐bisphosphate (Fru‐2,6‐P2) to fructose 6‐phosphate (Fru‐6‐P) and Pi has been identified and purified from plants, specifically the cytosolic fraction of spinach leaf parenchyma cells. Partially purified preparations of the enzyme, designated fructose 2,6‐bisphosphatase (Fru‐2,6‐P2ase), were inhibited by products of the reaction (i.e., Pi and Fru‐6‐P) but showed no response to a protein phosphorylation system known to inhibit the corresponding enzyme in mammalian cells. Fru‐2,6‐P2ase co‐purified with fructose 6‐phosphate,2‐kinase, the enzyme catalyzing the synthesis of Fru‐2,6‐P2. The observed pattern of regulation of the enzymes functional in the synthesis and breakdown of Fru‐2,6‐P2 reinforces the conclusion that chloroplasts play a role in controlling cytosolic carbon processing in leaves.
TI - A product‐regulated fructose 2,6‐bisphosphatase occurs in green leaves
JF - FEBS Letters
DO - 10.1016/0014-5793(83)81057-6
DA - 1983-10-03
UR - https://www.deepdyve.com/lp/wiley/a-product-regulated-fructose-2-6-bisphosphatase-occurs-in-green-leaves-LE5mt5hAM0
SP - 103
EP - 106
VL - 162
IS - 1
DP - DeepDyve
ER -