TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on October 21, 2021 - Published by Cold Spring Harbor Laboratory Press Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity 1,2 1,2,4 1,2,4 1,2,3,5 Qian Yang, Ken Inoki, Tsuneo Ikenoue, and Kun-Liang Guan 1 2 Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA; Institute of Gerontology, University of Michigan, Ann Arbor, Michigan 48109, USA Target of rapamycin (TOR) is an evolutionally conserved protein kinase in eukaryotes and a central cell growth controller. TOR exists in two distinct complexes, termed TORC1 and TORC2. Mammalian TORC2 has recently been shown to possess kinase activity toward the C-terminal hydrophobic site of Akt/PKB. Here, we report that Sin1 is an essential component of TORC2 but not of TORC1, and functions similarly to Rictor, the defining member of TORC2, in complex formation and kinase activity. Knockdown of Sin1decreases Akt phosphorylation in both Drosophila and mammalian cells and diminishes Akt function in vivo. It also disrupts the interaction between Rictor and mTOR. Furthermore, Sin1 is required for TORC2 kinase activity in vitro. Disruption of the Rictor gene in mice results 
TI - Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity
JF - Genes & Development
DO - 10.1101/gad.1461206
DA - 2006-10-15
UR - https://www.deepdyve.com/lp/unpaywall/identification-of-sin1-as-an-essential-torc2-component-required-for-Lt8lbf7YLy
DP - DeepDyve
ER -