TY - JOUR
AU - Furner, Ian J.
AB - Ninety‐eight independent transformed (T1) Arabidopsis plants were generated, containing additional copies of the chalcone synthase (CHS) gene. Three T2 generation families (A, B and C) were found that showed reduced anthocyanin biosynthesis, consistent with homology‐ dependent gene silencing of CHS. Clonal sectors of tissue showing CHS silencing were seen in the early generations. Affected individuals in family A showed only slight silencing, in family C such plants were almost completely silenced, and in family B affected individuals were intermediate. Plants homozygous for a single silencing insert were isolated from each family. Plants homozygous or hemizygous for insert A showed variable penetrance and expressivity of silencing. Self‐fertilization of plants hemizygous for the B and C‐inserts suggested that these CHS‐silencing inserts each behave as single Mendelian dominant traits. The CHS mRNA of the C‐insert homozygotes was reduced to undetectable levels.
TI - Genetics of homology‐dependent gene silencing in Arabidopsis; a role for methylation
JF - The Plant Journal
DO - 10.1046/j.1365-313X.1997.12040791.x
DA - 1997-10-01
UR - https://www.deepdyve.com/lp/wiley/genetics-of-homology-dependent-gene-silencing-in-arabidopsis-a-role-MLH1OOI08j
SP - 791
EP - 804
VL - 12
IS - 4
DP - DeepDyve
ER -