TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on October 25, 2021 - Published by Cold Spring Harbor Laboratory Press Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells Adrian R. Krainer, ~ Greg C. Conway, and Diane Kozak Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 USA SF2, an activity necessary for 5' splice site cleavage and lariat formation during pre-mRNA splicing in v/tro, has been purified to near homogeneity from HeLa cells. The purest fraction contains only two related polypeptides of 33 kD. This fraction is sufficient to complement an SI00 fraction, which contains the remaining splicing factors, to splice several pre-mRNAs. The optimal amount of SF2 required for efficient splicing depends on the pre-mRNA substrate. SF2 is distinct from the hnRNP A1 and U1 snRNP A polypeptides, which are similar in size. Endogenous hnRNA copurifies with SF2, but this activity does not appear to have an essential RNA component. SF2 appears to be necessary for the assembly or stabilization of the earliest specific prespliceosome complex, although in the absence of other components, it can bind RNA in a nonspecific manner. SF2 copurifies with an activity that promotes the annealing of complementary RNAs. Thus, SF2 may promote specific 
TI - Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells.
JF - Genes & Development
DO - 10.1101/gad.4.7.1158
DA - 1990-07-01
UR - https://www.deepdyve.com/lp/unpaywall/purification-and-characterization-of-pre-mrna-splicing-factor-sf2-from-MTa43gHN3C
DP - DeepDyve
ER -