TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on December 15, 2021 - Published by Cold Spring Harbor Laboratory Press Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast 1,2 1 1,3 J. Bryan McNeil, Helga Agah, and David Bentley Amgen Institute, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada We investigated whether the multisubunit holoenzyme complex of RNA polymerase II (Pol II) and mediator is universally required for transcription in budding yeast. DCTD Pol II lacking the carboxy-terminal domain of the large subunit cannot assemble with mediator but can still transcribe the CUP1 gene. CUP1 transcripts made by DCTD Pol II initiated correctly and some extended past the normal poly(A) site yielding a novel dicistronic mRNA. Most CUP1 transcripts made by DCTD Pol II were degraded but could be stabilized by deletion of the XRN1 gene. Unlike other genes, transcription of CUP1 and HSP82 also persisted after inactivation of the CTD kinase Kin28 or the mediator subunit Srb4. The upstream-activating sequence (UAS) 2+ of the CUP1 promoter was sufficient to drive Cu inducible transcription without Srb4 and heat shock inducible transcription without the CTD. We conclude that the Pol II holoenzyme is not essential for all UAS-dependent activated transcription in 
TI - Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast
JF - Genes & Development
DO - 10.1101/gad.12.16.2510
DA - 1998-08-15
UR - https://www.deepdyve.com/lp/unpaywall/activated-transcription-independent-of-the-rna-polymerase-ii-N38c3S0MtY
DP - DeepDyve
ER -