TY - JOUR
AU1 - Sven, Michel
AU2 - Liming, Liang
AU3 - Martin, Depner
AU4 - Norman, Klopp
AU5 - Andreas, Ruether
AU6 - Ashish, Kumar
AU7 - Michaela, Schedel
AU8 - Christian, Vogelberg
AU9 - Erika, von Mutius
AU1 - Andrea, von Berg
AU1 - Albrecht, Bufe
AU1 - Ernst, Rietschel
AU1 - Andrea, Heinzmann
AU1 - Otto, Laub
AU1 - Burkhard, Simma
AU1 - Thomas, Frischer
AU1 - Jon, Genuneit
AU1 - Ivo G., Gut
AU1 - Stefan, Schreiber
AU2 - Mark, Lathrop
AU2 - Thomas, Illig
AU2 - Michael, Kabesch
AB - The first genome wide association study (GWAS) for childhood asthma identified a novel major susceptibility locus on chromosome 17q21 harboring the ORMDL3 gene, but the role of previous asthma candidate genes was not specifically analyzed in this GWAS. We systematically identified 89 SNPs in 14 candidate genes previously associated with asthma in >3 independent study populations. We re-genotyped 39 SNPs in these genes not covered by GWAS performed in 703 asthmatics and 658 reference children. Genotyping data were compared to imputation data derived from Illumina HumanHap300 chip genotyping. Results were combined to analyze 566 SNPs covering all 14 candidate gene loci. Genotyped polymorphisms in ADAM33 , GSTP1 and VDR showed effects with p-values <0.0035 (corrected for multiple testing). Combining genotyping and imputation, polymorphisms in DPP10 , EDN1 , IL12B , IL13 , IL4 , IL4R and TNF showed associations at a significance level between p = 0.05 and p = 0.0035. These data indicate that (a) GWAS coverage is insufficient for many asthma candidate genes, (b) imputation based on these data is reliable but incomplete, and (c) SNPs in three previously identified asthma candidate genes replicate in our GWAS population with significance after correction for multiple testing in 14 genes.
TI - Unifying Candidate Gene and GWAS Approaches in Asthma
JF - PLoS ONE
DO - 10.1371/journal.pone.0013894
DA - 2010-11-12
UR - https://www.deepdyve.com/lp/public-library-of-science-plos-journal/unifying-candidate-gene-and-gwas-approaches-in-asthma-NlSPsn0AdP
VL - 5
IS - 11
DP - DeepDyve
ER -