TY - JOUR
AU1 - Kinoshita, Kazuhisa
AU2 - Noetzel, Tim L.
AU3 - Pelletier, Laurence
AU4 - Mechtler, Karl
AU5 - Drechsel, David N.
AU6 - Schwager, Anne
AU7 - Lee, Mike
AU8 - Raff, Jordan W.
AU9 - Hyman, Anthony A.
AB - Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.
TI - Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
JF - The Journal of Cell Biology
DO - 10.1083/jcb.200503023
DA - 2005-09-26
UR - https://www.deepdyve.com/lp/pubmed-central/aurora-a-phosphorylation-of-tacc3-maskin-is-required-for-centrosome-OfOO44c15H
SP - 1047
EP - 1055
VL - 170
IS - 7
DP - DeepDyve
ER -