TY - JOUR AU1 - Kinoshita, Kazuhisa AU2 - Noetzel, Tim L. AU3 - Pelletier, Laurence AU4 - Mechtler, Karl AU5 - Drechsel, David N. AU6 - Schwager, Anne AU7 - Lee, Mike AU8 - Raff, Jordan W. AU9 - Hyman, Anthony A. AB - Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. TI - Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis JF - The Journal of Cell Biology DO - 10.1083/jcb.200503023 DA - 2005-09-26 UR - https://www.deepdyve.com/lp/pubmed-central/aurora-a-phosphorylation-of-tacc3-maskin-is-required-for-centrosome-OfOO44c15H SP - 1047 EP - 1055 VL - 170 IS - 7 DP - DeepDyve ER -