TY - JOUR
AU1 - van Doorn, Leen-Jan
AU2 - Molijn, Anco
AU3 - Kleter, Bernhard
AU4 - Quint, Wim
AU5 - Colau, Brigitte
AB - Highly Effective Detection of Human Papillomavirus 16 and 18 DNA by a Testing Algorithm Combining Broad-Spectrum and Type-Specific PCR Leen-Jan van Doorn 1 , * , Anco Molijn 1 , Bernhard Kleter 1 , Wim Quint 1 and Brigitte Colau 2 1 DDL Diagnostic Laboratory, Fonteijnenburghlaan 5, Voorburg, The Netherlands 2 GSK Biologicals, Rue de l'Institut 89, Rixensart, Belgium ABSTRACT The use of a single broad-spectrum human papillomavirus (HPV) DNA-based PCR test may fail to detect lower concentrations of HPV DNA due to competition between different genotypes in mixed infections. To improve HPV detection by PCR, broad-spectrum and type-specific (TS) PCRs were combined, with a focus on HPV-16 and HPV-18. Cervical and cervicovaginal cell samples were obtained from 1,113 healthy women (age range, 15 to 25 years) participating in an HPV-16/HPV-18 candidate vaccine efficacy trial. These samples were tested by a broad-spectrum SPF 10 PCR-DNA enzyme immunoassay, followed by a primer SPF 10 -based line probe assay (SPF 10 LiPA), and HPV-16- and HPV-18-TS PCRs. The results for the majority of the HPV-16/18 SPF 10 LiPA-positive samples were confirmed by TS-PCR (kappa values, 0.775 for HPV-16 and 0.785 for HPV-18). However, TS PCR revealed additional positive samples among those that contained other HPV genotypes due to competition. Conversely, SPF 10 LiPA identified HPV-16 or -18 in samples that remained negative by TS PCR as a result of sampling variation. Analysis of follow-up samples from more than 1,000 women confirmed that the combination of SPF 10 -LiPA with additional HPV-16- and HPV-18-TS PCR diminishes the rate of false-negative diagnosis. The combination of broad-spectrum and TS PCRs resulted in a novel testing algorithm. This combination of assays is more accurate than either method alone, and the novel algorithm offers a highly accurate and effective method for the analysis of HPV infections.
TI - Highly Effective Detection of Human Papillomavirus 16 and 18 DNA by a Testing Algorithm Combining Broad-Spectrum and Type-Specific PCR
JF - Journal of Clinical Microbiology
DO - 10.1128/JCM.00539-06
DA - 2006-09-01
UR - https://www.deepdyve.com/lp/american-society-for-microbiology/highly-effective-detection-of-human-papillomavirus-16-and-18-dna-by-a-PF701iISCY
SP - 3292
VL - 44
IS - 9
DP - DeepDyve
ER -