TY - JOUR
AU1 - Rajeevan, Mangalathu
AU2 - Bassett, Carole
AU3 - Hughes, D.
AB - To study the control of enhanced synthesis of enzymes associated with C4 photosynthesis relative to non-C4 plants, we investigated the expression of NADP-malic enzyme (NADP-ME) in different photosynthetic types ofFlaveria. Complementary DNA clones encoding NADP-ME were constructed using poly(A)+ RNA from leaves ofFlaveria trinervia (C4) andF. linearis (C3–C4) and identified by homology to a cDNA clone (500 bp) encoding NADP-ME from maize (Zea mays L. [39]). The sequence of one clone from each species was determined. TheFlaveria clones were 90% homologous over a 564 nucleotide region encoding the carboxy terminal end of the derived polypeptide; sequence similarity to the maize transcript in this region was 71%. BothFlaveria clones detected a 2/3 kb transcript by hybridization to poly(A)+ RNA from expanding leaves ofF. trinervia, F. linearis andF. pringlei (C3). The level of transcripts paralleled previously observed NADP-ME activity and abundance differences determined in these species, suggesting that control of the expression of NADP-ME in different photosynthetic types is predominantly at the transcriptional/post-transcriptional level. Southern analysis of genomic DNAs fromF. trinervia, F. linearis andF. pringlei indicated a low copy number for this gene in all three species.
TI - Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves ofFlaveria: transcript abundance distinguishes C3, C3–C4 and C4 photosynthetic types
JF - Plant Molecular Biology
DO - 10.1007/BF00040632
DA - 2004-04-15
UR - https://www.deepdyve.com/lp/springer-journals/isolation-and-characterization-of-cdna-clones-for-nadp-malic-enzyme-Q3NP28MhtB
SP - 371
EP - 383
VL - 17
IS - 3
DP - DeepDyve
ER -