TY - JOUR
AU1 - Barabino, S M
AU2 - Hübner, W
AU3 - Jenny, A
AU4 - Minvielle-Sebastia, L
AU5 - Keller, W
AU6 - 
AB - Downloaded from genesdev.cshlp.org on October 9, 2021 - Published by Cold Spring Harbor Laboratory Press The 30-kD subunit of mammalian . . cleavage and polyadenylation specificity factor and its yeast .homolog are RNA-binding zinc finger proteins Silvia M.L. Barabino, Wolfgang Hiibner, Andreas Jenny, 1 Lionel Minvielle-Sebastia, and Walter Keller 2 Department of Cell Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists of four subunits of 160, 100, 73, and 30 kD. Here we report the isolation and characterization of a cDNA encoding the 30-kD polypeptide. Antibodies raised against this protein inhibit cleavage and polyadenylation and coimmunoprecipitate the other CPSF subunits. The protein sequence contains five C3H-zinc-finger repeats and a putative RNA-binding zinc knuckle motif at the carboxyl terminus. Consistent with this observation, the in vitro translated 30-kD protein binds RNA polymers with a distinct preference for poly(U). In addition, an essential S. cerevisiae gene, YTH1, was cloned which is 40% identical to CPSF 30K at the protein level. Extracts prepared from a conditional ythl mutant have normal cleavage activity, but fail to polyadenylate the upstream cleavage product. Efficient polyadenylation activity can be 
TI - The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.
JF - Genes & Development
DO - 10.1101/gad.11.13.1703
DA - 1997-07-01
UR - https://www.deepdyve.com/lp/unpaywall/the-30-kd-subunit-of-mammalian-cleavage-and-polyadenylation-TzvmSdYdoJ
DP - DeepDyve
ER -