TY - JOUR
AU1 - Gäher, Martin
AU2 - Einsiedler, Kerstin
AU3 - Crass, Torsten
AU4 - Bautsch, Wilfried
AB - A physical map of the chromosome of Neisseria meningitidis B1940 has been constructed by one‐ and two‐dimensional pulsed‐field gel electrophoresis techniques. Complete macrorestriction maps for the enzymes NheI (16 sites), SgfI (13 sites), SfiI (11 sites) and I‐CeuI (4 sites), as well as a partial restriction map for the restriction enzyme SpeI (15 of c. 28 sites) could be established. Altogether, 59 restriction sites were mapped on a single circular chromosome of 2.3 Mbp. By restriction endonuclease digestion and Southern hybridization of cloned genetic markers, 39 genetic loci were assigned to this map. Comparison with the metabolic maps of Neisseria gonorrhoeae MS11‐N198 and FA1090 revealed a high degree of conservation in the arrangement of gene loci among these two species, although four out of 24 genetic loci are located at different chromosomal positions, indicating several genomic rearrangements.
TI - A physical and genetic map of Neisseria meningitidis B1940
JF - Molecular Microbiology
DO - 10.1046/j.1365-2958.1996.416901.x
DA - 1996-01-01
UR - https://www.deepdyve.com/lp/wiley/a-physical-and-genetic-map-of-neisseria-meningitidis-b1940-UkMEH5Nw01
SP - 249
VL - 19
IS - 2
DP - DeepDyve
ER -